I am looking for a Dio3 peptide that I can inject in vivo to produce a biological effect. Can you provide information or confirm whether the Dio3 peptide you have would be effective as a biological agent in vivo?

Name: DIO3 Antibody [Janelia Fluor&#174; 585]
Brand: Novus Biologicals
SKU: NBP1-05767JF585
Price: 499 USD

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Accepted Answer

I have pulled up information on the Dio3 peptides and recombinant proteins that we have available. Unfortunately I would not expect any of them to be biologically active and capable of producing a response when injected in vivo. The two peptides we carry are simply intended to be used for negative control competition assays to block signal of the primary and determine what is specific and non-specific as far as signal is concerned. The other two recombinant proteins are ones we distribute for a Taiwanese company called Abnova and they should not be biologically active based on the cell free wheat germ system employed to synthesize them. We will typically list our active proteins with an indication of such on our datasheet if it has been tested and usually the application of functional listed.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Ha, RbSpecies Glossary
Applications	WB, Flow, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Janelia Fluor 585

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP1-05767AF350

Alexa Fluor 405 NBP1-05767AF405

Alexa Fluor 488 NBP1-05767AF488

Alexa Fluor 532 NBP1-05767AF532

Alexa Fluor 594 NBP1-05767AF594

Alexa Fluor 647 NBP1-05767AF647

Alexa Fluor 700 NBP1-05767AF700

Alexa Fluor 750 NBP1-05767AF750

Allophycocyanin NBP1-05767APC

Biotin NBP1-05767B

CoraFluor 1 NBP1-05767CL1

DyLight 350 NBP1-05767UV

DyLight 405 NBP1-05767V

DyLight 488 NBP1-05767G

DyLight 550 NBP1-05767R

DyLight 594 NBP1-05767DL594

DyLight 650 NBP1-05767C

DyLight 680 NBP1-05767FR

DyLight 755 NBP1-05767IR

FITC NBP1-05767F

HRP NBP1-05767H

Janelia Fluor 525 NBP1-05767JF525

Janelia Fluor 549 NBP1-05767JF549

Janelia Fluor 635 NBP1-05767JF635

Janelia Fluor 646 NBP1-05767JF646

Janelia Fluor 669 NBP1-05767JF669

PE NBP1-05767PE

PerCP NBP1-05767PCP

Unconjugated NBP1-05767

mFluor Violet 450 SE NBP1-05767MFV450

mFluor Violet 500 SE NBP1-05767MFV500

mFluor Violet 610 SE NBP1-05767MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NBP1-05767

BSA Free NBP1-05767SS

BSA Free NBP1-05767

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-05767AF350
Alexa Fluor 405	NBP1-05767AF405
Alexa Fluor 488	NBP1-05767AF488
Alexa Fluor 532	NBP1-05767AF532
Alexa Fluor 594	NBP1-05767AF594
Alexa Fluor 647	NBP1-05767AF647
Alexa Fluor 700	NBP1-05767AF700
Alexa Fluor 750	NBP1-05767AF750
Allophycocyanin	NBP1-05767APC
Biotin	NBP1-05767B
CoraFluor 1	NBP1-05767CL1
DyLight 350	NBP1-05767UV
DyLight 405	NBP1-05767V
DyLight 488	NBP1-05767G
DyLight 550	NBP1-05767R
DyLight 594	NBP1-05767DL594
DyLight 650	NBP1-05767C
DyLight 680	NBP1-05767FR
DyLight 755	NBP1-05767IR
FITC	NBP1-05767F
HRP	NBP1-05767H
Janelia Fluor 525	NBP1-05767JF525
Janelia Fluor 549	NBP1-05767JF549
Janelia Fluor 635	NBP1-05767JF635
Janelia Fluor 646	NBP1-05767JF646
Janelia Fluor 669	NBP1-05767JF669
PE	NBP1-05767PE
PerCP	NBP1-05767PCP
Unconjugated	NBP1-05767
mFluor Violet 450 SE	NBP1-05767MFV450
mFluor Violet 500 SE	NBP1-05767MFV500
mFluor Violet 610 SE	NBP1-05767MFV610

DIO3 Antibody [Janelia Fluor® 585]

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Immunogen	Synthetic peptide made to an internal portion of rat DIO3 (within residues 250-300). [Swiss-Prot# P49897]
Localization	Cell membrane; Single-pass type II membrane protein
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	DIO3
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Flow Cytometry Immunocytochemistry/ Immunofluorescence Immunohistochemistry Immunohistochemistry-Frozen Immunohistochemistry-Paraffin Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Immunogen affinity purified