![Western Blot: DFF40/CAD Antibody [NBP1-76948] - Analysis of DFF40/CAD in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with anti-DFF40/CAD (I18) using NBP1-76948 at 1:500 dilution.](http://images.novusbio.com/fullsize/DFF40-CAD-Antibody-Western-Blot-NBP1-76948-img0001.jpg "Western Blot: DFF40/CAD Antibody [NBP1-76948] - Analysis of DFF40/CAD in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with anti-DFF40/CAD (I18) using NBP1-76948 at 1:500 dilution.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 18 amino acids near the center of murine CAD. The immunogen is located within amino acids 130 - 180 of DFF40. Amino Acid Squence: GLESRFRNKSGYLRYSC |
| Predicted Species | Bovine (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DFFB |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | A 40 kDa band can be detected in Western Blot. |
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| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for DFF40/CAD Antibody (NBP1-76948)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: DFF40/CAD Antibody [NBP1-76948] - Staining of DFF40 in K562 cells with DFF40 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/DFF40-CAD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76948-img0003.jpg "Immunocytochemistry/Immunofluorescence: DFF40/CAD Antibody [NBP1-76948] - Staining of DFF40 in K562 cells with DFF40 antibody at 20 ug/ml.")
![Immunocytochemistry/Immunofluorescence: DFF40/CAD Antibody [NBP1-76948] - Jurkat cells with DFF40 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/DFF40-CAD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76948-img0002.jpg "Immunocytochemistry/Immunofluorescence: DFF40/CAD Antibody [NBP1-76948] - Jurkat cells with DFF40 antibody at 5 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948] - Immunofluorescence of DFF40/CAD in K562 cells with DFF40/CAD antibody at 20 u/mL.Green: DFF40/CAD Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76948_rabbit-polyclonal-dff40-cad-antibody-2711202420175131.gif "Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948] - Immunofluorescence of DFF40/CAD in K562 cells with DFF40/CAD antibody at 20 u/mL.Green: DFF40/CAD Antibody Blue: DAPI staining")
![Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948] - Immunofluorescence of DFF40/CAD in K562 cells with DFF40/CAD antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp1-76948_rabbit-polyclonal-dff40-cad-antibody-2711202419562137.gif "Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948] - Immunofluorescence of DFF40/CAD in K562 cells with DFF40/CAD antibody at 20 u/ml.")
![Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948] - Immunofluorescence of DFF40/CAD in K562 cells with DFF40/CAD antibody at 20 u/mL.Green: DFF40/CAD Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76948_rabbit-polyclonal-dff40-cad-antibody-2711202420312916.gif "Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948] - Immunofluorescence of DFF40/CAD in K562 cells with DFF40/CAD antibody at 20 u/mL.Green: DFF40/CAD Antibody Blue: DAPI staining")
![Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948] - Immunofluorescence of DFF40/CAD in K562 cells with DFF40/CAD antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp1-76948_rabbit-polyclonal-dff40-cad-antibody-271120242010589.gif "Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948] - Immunofluorescence of DFF40/CAD in K562 cells with DFF40/CAD antibody at 20 u/ml.")
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot AIF Antibody [Unconjugated]](https://images.novusbio.com/images2/AIF_AF1457_Western_Blot_5113.jpg)
![Simple Western AIF Antibody [Unconjugated]](https://images.novusbio.com/images2/16334.jpg)
![Knockout Validated AIF Antibody [Unconjugated]](https://images.novusbio.com/images2/AIF_AF1457_Knockout_Validated_22786.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")