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DFF40/CAD Antibody [mFluor Violet 450 SE]

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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
mFluor Violet 450 SE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-76948AF350
Alexa Fluor 405
NBP1-76948AF405
Alexa Fluor 488
NBP1-76948AF488
Alexa Fluor 532
NBP1-76948AF532
Alexa Fluor 594
NBP1-76948AF594
Alexa Fluor 647
NBP1-76948AF647
Alexa Fluor 700
NBP1-76948AF700
Alexa Fluor 750
NBP1-76948AF750
Biotin
NBP1-76948B
CoraFluor 1
NBP1-76948CL1
DyLight 350
NBP1-76948UV
DyLight 405
NBP1-76948V
DyLight 488
NBP1-76948G
DyLight 550
NBP1-76948R
DyLight 594
NBP1-76948DL594
DyLight 650
NBP1-76948C
DyLight 680
NBP1-76948FR
DyLight 755
NBP1-76948IR
FITC
NBP1-76948F
HRP
NBP1-76948H
Janelia Fluor 525
NBP1-76948JF525
Janelia Fluor 549
NBP1-76948JF549
Janelia Fluor 646
NBP1-76948JF646
Janelia Fluor 669
NBP1-76948JF669
PerCP
NBP1-76948PCP
Unconjugated
NBP1-76948
mFluor Violet 500 SE
NBP1-76948MFV500
mFluor Violet 610 SE
NBP1-76948MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-76948
BSA Free
NBP1-76948
BSA Free
NBP1-76948SS
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DFF40/CAD Antibody [mFluor Violet 450 SE] Summary

Immunogen
Antibody was raised against a peptide corresponding to 18 amino acids near the center of murine CAD. The immunogen is located within amino acids 130 - 180 of DFF40. Amino Acid Squence: GLESRFRNKSGYLRYSC
Predicted Species
Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DFFB
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DFF40/CAD Antibody [mFluor Violet 450 SE]

  • CAD
  • CADCaspase-activated nuclease
  • Caspase-activated deoxyribonuclease
  • Caspase-activated DNase
  • CPAN
  • CPANDNA fragmentation factor 40 kDa subunit
  • DFF2
  • DFF40
  • DFF-40DFF40DFF2DNA fragmentation factor subunit beta
  • DFFB
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • EC 3.-

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DFF40/CAD Antibody
[mFluor Violet 450 SE ...
NBP1-76948MFV450
DFF40/CAD Antibody [mFluor Violet 450 SE]
Species: Hu, Mu, Rt, Bv
Applications: WB, ELISA, ICC/IF

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DFF40/CAD Antibody (NBP1-76948MFV450). (Showing 1 - 1 of 1 FAQ).

  1. Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?
    • CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DFFB