![Western Blot: Desmoglein-2 Antibody [NBP1-33374] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Desmoglein-2-Antibody-Western-Blot-NBP1-33374-img0016.jpg "Western Blot: Desmoglein-2 Antibody [NBP1-33374] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Desmoglein-2. The exact sequence is proprietary. |
| Localization | Cell membrane, Cell junction, desmosome |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DSG2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 122 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 1% BSA, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Desmoglein-2 Antibody (NBP1-33374)Find related products by research area.
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| Gene Symbol | DSG2 |
![Immunocytochemistry/Immunofluorescence: Desmoglein-2 Antibody [NBP1-33374] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Desmoglein-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33374-img0014.jpg "Immunocytochemistry/Immunofluorescence: Desmoglein-2 Antibody [NBP1-33374] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374] - Human colon cancer. Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Desmoglein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-33374-img0013.jpg "Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374] - Human colon cancer. Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: Desmoglein-2 Antibody [NBP1-33374] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein-2 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Desmoglein-2-Antibody-Western-Blot-NBP1-33374-img0012.jpg "Western Blot: Desmoglein-2 Antibody [NBP1-33374] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein-2 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374] - DU145 xenograft , using Desmoglein-2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Desmoglein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-33374-img0006.jpg "Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374] - DU145 xenograft , using Desmoglein-2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374] - Mouse skin. Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Desmoglein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-33374-img0011.jpg "Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374] - Mouse skin. Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")