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DAP Kinase 2 Antibody - BSA Free

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Western Blot: DAP Kinase 2 Antibody [NBP1-77291] - Analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody at 1 ug/mL.
Immunohistochemistry: DAP Kinase 2 Antibody - BSA Free [NBP1-77291] - Immunohistochemistry of DAP Kinase 2 in mouse spleen cells with DAP Kinase 2 antibody at 2 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price

DAP Kinase 2 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2. This antigenic peptide is identical to the corresponding amino acids of mouse origin (1,2). The immunogen is located within the last 50 amino acids of DAPK2. Amino Acid Squence: ARRKALHPRRRSSTS
Specificity
DAPK2 has no cross responses to DAPK1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DAPK2
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 1 ug/ml
Application Notes
An approximately 42 kDa band can be detected in Western Blot.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
DAP Kinase 2 Peptide (NBP1-77291PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for DAP Kinase 2 Antibody - BSA Free

  • DAP Kinase 2
  • DAPK2
  • DAP-kinase-related protein 1 beta isoform
  • DAP-kinase-related protein 1
  • death-associated protein kinase 2
  • DRP1
  • DRP-1
  • DRP-1MGC119312
  • EC 2.7.11
  • EC 2.7.11.1

Background

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNgamma induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DAPK2
Uniprot