DAP Kinase 2 Antibody - BSA Free Summary
Immunogen |
Antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2. This antigenic peptide is identical to the corresponding amino acids of mouse origin (1,2). The immunogen is located within the last 50 amino acids of DAPK2. Amino Acid Squence: ARRKALHPRRRSSTS |
Specificity |
DAPK2 has no cross responses to DAPK1. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
DAPK2 |
Purity |
Peptide affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:100-1:2000
- Immunohistochemistry 2 ug/ml
- Immunohistochemistry-Paraffin 2 ug/ml
- Western Blot 1 ug/ml
|
Application Notes |
An approximately 42 kDa band can be detected in Western Blot. |
Theoretical MW |
42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Control Peptide |
|
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.02% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Peptide affinity purified |
Alternate Names for DAP Kinase 2 Antibody - BSA Free
Background
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNgamma induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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