Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ha, Ma, Sh, Ch(-), Xp(-)Species Glossary |
Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
Clone | SPM268 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | Azide and BSA Free |
Concentration | 1.0 mg/ml |
Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A or G chromatography supplied in 10 mM PBS, pH 7.4 WITHOUT BSA & azide. Antibody with azide - store at 2 to 8C. Antibody is stable for 24 months. |
Immunogen | Cytoskeletal preparation from HeLa cells |
Localization | Cytoplasmic |
Specificity | It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | KRT5 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT. Optimal dilution for a specific application should be determined. |
Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
Buffer | 10 mM PBS, pH 7.4 |
Preservative | No Preservative |
Concentration | 1.0 mg/ml |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 5/8 Antibody (NBP2-47823)Find related products by research area.
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Gene Symbol | KRT5 |