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DDT Antibody (OTI1H3)

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Western Blot: DDT Antibody (1H3) [NBP2-45677] - Analysis of extracts (35ug) from 9 different cell lines by using DDT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; ...read more
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: DDT Antibody (1H3) [NBP2-45677] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDT.
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, IHC
Clone
OTI1H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DDT Antibody (OTI1H3) Summary

Immunogen
Full length human recombinant protein of human DDT(NP_001346) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DDT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200 - 8000
Theoretical MW
12.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDT Antibody (OTI1H3)

  • DDCT
  • D-Dopachrome Decarboxylase
  • D-dopachrome tautomeraseDDCT
  • DDT
  • DOPD
  • EC 4.1.1.84
  • MIF2
  • Phenylpyruvate Tautomerase II

Background

D-dopachrome decarboxylase, also known as D-dopachrome tautomerase (DDT), phenylpyruvate tautomerase II and DDCT, is a member of the migration inhibitory factor (MIF) family. DDT, via tautomerization and decarboxylation, converts D-dopachrome into 5,6-dihydroxyindole (DHI). DDT may also play a role in melanin biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DDT