![Western Blot: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Western blot analysis of Cytokeratin 5/75 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Go](http://images.novusbio.com/fullsize/Cytokeratin-5-75-Antibody-SC62-04-Western-Blot-NBP2-67586-img0008.jpg "Western Blot: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Western blot analysis of Cytokeratin 5/75 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Go")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | SC62-04 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within human Cytokeratin 5/75 aa 70-110. (SwissProt: P13647 Human) |
| Localization | Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KRT5 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 5/75 Antibody (NBP2-67586)Find related products by research area.
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| Gene Symbol | KRT5 |
![Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5/75 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-5-75-Antibody-SC62-04-Immunohistochemistry-Paraffin-NBP2-67586-img0007.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5/75 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of Cytokeratin 5 on A431 cell lysates using anti-Cytokeratin 5 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-5-75-Antibody-SC62-04-Western-Blot-NBP2-67586-img0004.jpg "Western Blot: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of Cytokeratin 5 on A431 cell lysates using anti-Cytokeratin 5 antibody at 1/1,000 dilution.")
![Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-5-75-Antibody-SC62-04-Immunohistochemistry-Paraffin-NBP2-67586-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of paraffin-embedded human liver cancer tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-5-75-Antibody-SC62-04-Immunohistochemistry-Paraffin-NBP2-67586-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of paraffin-embedded human liver cancer tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-5-75-Antibody-SC62-04-Immunohistochemistry-Paraffin-NBP2-67586-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cytokeratin 5/75 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-5-75-Antibody-SC62-04-Immunohistochemistry-Paraffin-NBP2-67586-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cytokeratin 5/75 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Cytokeratin 5/75 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-5-75-Antibody-SC62-04-Immunohistochemistry-Paraffin-NBP2-67586-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Cytokeratin 5/75 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
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