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Cystatin D/Cst5 Antibody (206006)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, B/N
Clone
206006
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Cystatin D/Cst5 Antibody (206006) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin D
Arg28-Val142
Accession # P28325
Specificity
Detects human Cystatin D in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cystatins A, B, C, E/M, S, SA, SN, rhKininogen, rhFetuins A, B, or rhHPRG is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CST5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Neutralization 2.8 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using MAB1202.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin D/Cst5 Antibody (206006)

  • CST5
  • CSTD
  • cystatin 5
  • Cystatin D
  • Cystatin-5
  • cystatin-D
  • cysteine-proteinase inhibitor
  • MGC71922

Background

Cystatin D is a member of family 2 of the cystatin superfamily (1). In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg46 and Cys46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and cathepsins B, H, L and S (2). The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.12‑1.9 μM) and has been suggested to play a protective role against proteases present in the oral cavity (3).

  1. Freije, J.P. et al. (1993) J. Biol. Chem. 268:15737.
  2. Balbin, M. et al. (1994) J. Biol. Chem. 269:23156.
  3. Collins, A.R. and A. Grubb (1998) Oral Microbiol. Immunol. 13:59.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cystatin D/Cst5 Antibody (MAB1202)(1)

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Bioinformatics

Gene Symbol CST5
Uniprot