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Cystatin S Antibody (OTI2H10) - Azide and BSA Free

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Western Blot: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cystatin S (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cystatin S.
Flow Cytometry: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by ...read more
Western Blot: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - Analysis of extracts (10ug) from HeLa cell line by using anti-CST4 monoclonal antibody at 1:200 dilution.
Immunocytochemistry/ Immunofluorescence: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - Staining of HeLa cells using anti-Cystatin S mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, ICC/IF, CyTOF-ready
Clone
OTI2H10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Cystatin S Antibody (OTI2H10) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human CST4(NP_001890) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CST4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Western Blot 1:2000
Theoretical MW
14.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for Cystatin S Antibody (OTI2H10) - Azide and BSA Free

  • CST4
  • cystatin 4
  • Cystatin S
  • Cystatin-4
  • cystatin-S
  • cystatin-SA-III
  • MGC71923
  • Salivary acidic protein 1

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CST4