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CYLD Antibody

Images

 
Western Blot: CYLD Antibody [NB100-78600] - A. C8D30 whole cell lysate/extract. B. NIH-3T3 whole cell lysate/extract. C. BCL-1 whole cell lysate/extract. D. Raw264.7 whole cell lysate/extract. E. C2C12 whole cell ...read more
Western Blot: CYLD Antibody [NB100-78600] - A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGECYLD antibody dilution: 1:500.
Western Blot: CYLD Antibody [NB100-78600] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody (NB100-78600) diluted at 1:1000. The HRP-conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CYLD Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CYLD. The exact sequence is proprietary.
Localization
Cytoplasmic, perinuclear region
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYLD
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for CYLD Antibody

  • BRSS
  • CDMT
  • CYLD
  • CYLD1
  • CYLD1MFT
  • CYLDI
  • cylindromatosis (turban tumor syndrome)
  • Deubiquitinating enzyme CYLD
  • EAC
  • EC 3.1.2.15
  • EC 3.4.19.12
  • FLJ20180
  • FLJ31664
  • KIAA0849FLJ78684
  • MFT1
  • probable ubiquitin carboxyl-terminal hydrolase CYLD
  • SBS
  • TEM
  • ubiquitin carboxyl-terminal hydrolase CYLD
  • ubiquitin specific peptidase like 2
  • ubiquitin thioesterase CYLD
  • Ubiquitin thiolesterase CYLD
  • Ubiquitin-specific-processing protease CYLD
  • USPL2

Background

CYLinDromatosis 1 (CYLD1) was first identified by Bignell et al. in 2000. which containing mutations responsible for cylindromatosis (CYLD; 132700), and coded by 956 aa, and a splice variant of 953 aa. The CYLD1 protein has 3 cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, which are found in proteins that coordinate the attachment of organelles to microtubules, and also with a catalytic domain of ubiquitin carboxy-terminal hydrolases homology. CYLD1 expressed major in fetal brain, testis, and skeletal muscle, and at minor level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary, and lung. CYLD enhances the activitation of NF-kappa-B, TRAF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CYLD Antibody (NB100-78600) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CYLD
Entrez
Uniprot