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CXCR2/IL-8RB Antibody (866614R) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with either (A) rat CXCR2/IL-8 RB or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB8110R) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with either (A) rat CXCR2/IL-8 RB or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB8110R) ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, CyTOF-ready
Clone
866614R
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB8110RU-100UG
Alexa Fluor 405
FAB8110RV-100UG
Alexa Fluor 488
FAB8110RG-100UG
Alexa Fluor 532
FAB8110RX
Alexa Fluor 594
FAB8110RT-100UG
Alexa Fluor 647
FAB8110RR-100UG
Alexa Fluor 700
FAB8110RN-100UG
Alexa Fluor 750
FAB8110RS-100UG
Allophycocyanin
FAB8110RA
Allophycocyanin/Cy7
FAB8110RAPCCY7
Biotin
FAB8110RB
CoraFluor 1
FAB8110RCL1
DyLight 350
FAB8110RD
DyLight 405
FAB8110RE
DyLight 488
FAB8110RK
DyLight 550
FAB8110RL
DyLight 594
FAB8110RM
DyLight 650
FAB8110RW
DyLight 680
FAB8110RY
DyLight 755
FAB8110RZ
FITC
FAB8110RF
HRP
FAB8110RH
Janelia Fluor 525
FAB8110RJF525
Janelia Fluor 549
FAB8110RI
Janelia Fluor 585
FAB8110RJF585
Janelia Fluor 635
FAB8110RJF635
Janelia Fluor 646
FAB8110RJ
Janelia Fluor 669
FAB8110RJF669
PE
FAB8110RP
PE/Atto594
FAB8110RPEATT594
PE/Cy5.5
FAB8110RPECY55
PE/Cy7
FAB8110RPECY7
PerCP
FAB8110RC
mFluor Violet 450 SE
FAB8110RMFV450
mFluor Violet 500 SE
FAB8110RMFV500
mFluor Violet 610 SE
FAB8110RMFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8110R-SP
Carrier Free
FAB8110RV-100UG
Carrier Free
FAB8110RU-100UG
Carrier Free
FAB8110RT-100UG
Carrier Free
FAB8110RS-100UG
Carrier Free
FAB8110RR-100UG
Carrier Free
FAB8110RG-100UG
Carrier Free
FAB8110RN-100UG
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CXCR2/IL-8RB Antibody (866614R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
NS0 mouse myeloma cell line transfected with rat CXCR2/IL-8 RB
Accession # P35407
Specificity
Detects rat CXCR2/IL-8 RB in direct ELISAs. In Flow Cytometry, stains HEK293 cells transfected with rat CXCR2/IL-8 RB, but does not stain non‑transfected cells.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR2/IL-8RB Antibody (866614R) [Unconjugated]

  • CD182 antigen
  • CD182
  • CDw128b
  • chemokine (C-X-C motif) receptor 2
  • chemokine (CXC) receptor 2
  • CMKAR2
  • C-X-C chemokine receptor type 2
  • CXCR2 gene for IL8 receptor type B
  • CXCR2
  • CXC-R2
  • CXCR-2
  • GRO/MGSA receptor
  • High affinity interleukin-8 receptor B
  • IL-8 RB
  • IL-8 receptor type 2
  • IL-8R B
  • IL8R2
  • IL8RA
  • IL8RB
  • interleukin 8 receptor B
  • interleukin 8 receptor type 2
  • interleukin-8 receptor type B

Background

CXCR2 is an approximately 40 kDa 7-transmembrane domain receptor for the ELR+ chemokines CXCL1, 2, 3, 5, 6, 7, 8 and MIF. CXCR2 is expressed on neutrophils, monocytes, eosinophils, basophils, mast cells, T cells, oligodendrocytes, airway smooth muscle cells, and vascular endothelial cells. It is additionally upregulated in several cancers. CXCR2 can associate into homodimers or heterodimers with CXCR1, CXCR4, CD74, or the delta Opioid Receptor. CXCR2 plays an important role in attracting immune cells to sites of inflammation followed by their adhesion and extravasation. It is also involved in angiogenesis, the development of inflammatory disorders, and cancer. Rat CXCR2 shares 71% and 86% amino acid sequence identity with human and mouse CXCR2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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