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CXCR2/IL-8RB Antibody (242216) [Unconjugated]

Images

 
HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouseCXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB2164) ...read more
CXCR2/IL‑8 RB was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB2164) at 5 µg/mL for 1 hour at room temperature followed by ...read more
Recombinant Mouse CXCL2/MIP‑2 (Catalog #452-M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready
Clone
242216
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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CXCR2/IL-8RB Antibody (242216) [Unconjugated] Summary

Immunogen
C6 rat glioma cell line transfected with mouse CXCR2
Met1-Leu359
Accession # P35343
Specificity
Stains mouse CXCR2 transfected cells but not CXCR1 transfectants.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CXCR2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • Neutralization 15-50 ug/mL
Publications
Read Publications using
MAB2164 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR2/IL-8RB Antibody (242216) [Unconjugated]

  • CD182 antigen
  • CD182
  • CDw128b
  • chemokine (C-X-C motif) receptor 2
  • chemokine (CXC) receptor 2
  • CMKAR2
  • C-X-C chemokine receptor type 2
  • CXCR2 gene for IL8 receptor type B
  • CXCR2
  • CXC-R2
  • CXCR-2
  • GRO/MGSA receptor
  • High affinity interleukin-8 receptor B
  • IL-8 RB
  • IL-8 receptor type 2
  • IL-8R B
  • IL8R2
  • IL8RA
  • IL8RB
  • interleukin 8 receptor B
  • interleukin 8 receptor type 2
  • interleukin-8 receptor type B

Background

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha , GRO beta , GRO gamma , NAP-2, and ENA-78.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR2/IL-8RB Antibody (MAB2164)(44)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Flow Cytometry, Functional Assay, IHC, IHC-Fr, In Vivo, Neutralization.


Filter By Application
Flow Cytometry
(12)
Functional Assay
(1)
IHC
(3)
IHC-Fr
(1)
In Vivo
(2)
Neutralization
(18)
All Applications
Filter By Species
Mouse
(37)
All Species
Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using MAB2164 Applications Species
Sawant, KV;Sepuru, KM;Penaranda, B;Lowry, E;Garofalo, RP;Rajarathnam, K; Chemokine Cxcl1-Cxcl2 heterodimer is a potent neutrophil chemoattractant Journal of leukocyte biology 2023-08-25 [PMID: 37625009] (Flow Cytometry, Mouse) Flow Cytometry Mouse
S Luo, C Liao, L Zhang, C Ling, X Zhang, P Xie, G Su, Z Chen, L Zhang, T Lai, J Tang METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling Cell Reports, 2023-03-14;42(3):112259. 2023-03-14 [PMID: 36920907] (Neutralization, Mouse) Neutralization Mouse
P Pavlidis, A Tsakmaki, E Pantazi, K Li, D Cozzetto, J Digby-Bell, F Yang, JW Lo, E Alberts, ACC Sa, U Niazi, J Friedman, AK Long, Y Ding, CD Carey, C Lamb, M Saqi, M Madgwick, L Gul, A Treveil, T Korcsmaros, TT Macdonald, GM Lord, G Bewick, N Powell Interleukin-22 regulates neutrophil recruitment in ulcerative colitis and is associated with resistance to ustekinumab therapy Nature Communications, 2022-10-03;13(1):5820. 2022-10-03 [PMID: 36192482] (In Vivo, Mouse) In Vivo Mouse
A Shimada, S Hasegawa-I Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice Toxicology reports, 2021-03-10;8(0):520-528. 2021-03-10 [PMID: 33747797] (IHC, Mouse) IHC Mouse
Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al. Cytotoxic T cells swarm by homotypic chemokine signalling eLife 10/13/2020 [PMID: 33046212]
K Ogawa, K Asano, S Yotsumoto, T Yamane, M Arita, Y Hayashi, H Harada, C Makino-Oka, H Fukuyama, K Kondo, T Yamasoba, M Tanaka Frontline Science:�Conversion of neutrophils into atypical Ly6G+ SiglecF+ immune cells with neurosupportive potential in olfactory neuroepithelium J. Leukoc. Biol., 2020-07-29;0(0):. 2020-07-29 [PMID: 32725843] (Flow Cytometry, Mouse) Flow Cytometry Mouse
RS Aquino, A Hayashida, PW Park Host syndecan-1 promotes listeriosis by inhibiting intravascular neutrophil extracellular traps PLoS Pathog., 2020-05-26;16(5):e1008497. 2020-05-26 [PMID: 32453780] (In Vivo, Mouse) In Vivo Mouse
Z Zhang, L Dong, A Jia, X Chen, Q Yang, Y Wang, Y Wang, R Liu, Y Cao, Y He, Y Bi, G Liu Glucocorticoids Promote the Onset of Acute Experimental Colitis and Cancer by Upregulating mTOR Signaling in Intestinal Epithelial Cells Cancers (Basel), 2020-04-11;12(4):. 2020-04-11 [PMID: 32290362] (Flow Cytometry, Mouse) Flow Cytometry Mouse
MY Park, HS Kim, HY Lee, BA Zabel, YS Bae Novel CD11b+Gr-1+Sca-1+ myeloid cells drive mortality in bacterial infection Sci Adv, 2020-01-22;6(4):eaax8820. 2020-01-22 [PMID: 32010784] (Flow Cytometry, Mouse) Flow Cytometry Mouse
M Mohammad, MT Nguyen, C Engdahl, M Na, A Jarneborn, Z Hu, A Karlsson, R Pullerits, A Ali, F Götz, T Jin The YIN and YANG of lipoproteins in developing and preventing infectious arthritis by Staphylococcus aureus PLoS Pathog., 2019-06-21;15(6):e1007877. 2019-06-21 [PMID: 31226163] (Neutralization, Mouse) Neutralization Mouse
Show All 44 Publications.

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Bioinformatics

Gene Symbol CXCR2
Uniprot