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CX3CL1/Fractalkine Antibody (81506) [Unconjugated]

 (10 Publications)
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Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND50 is typically 1-5 µg/mL.
Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N, CyTOF-ready, ELISA(Cap), Flow
Clone
81506
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC3652U-100UG
Alexa Fluor 405
IC3652V-100UG
Alexa Fluor 488
IC3652G-100UG
Alexa Fluor 532
FAB3652X
Alexa Fluor 594
IC3652T-100UG
Alexa Fluor 647
IC3652R-100UG
Alexa Fluor 700
IC3652N-100UG
Alexa Fluor 750
IC3652S-100UG
Allophycocyanin
FAB3652A
Biotin
FAB3652B
CoraFluor 1
FAB3652CL1
DyLight 350
FAB3652D
DyLight 405
FAB3652E
DyLight 488
FAB3652K
DyLight 550
FAB3652L
DyLight 594
FAB3652M
DyLight 650
FAB3652W
DyLight 680
FAB3652Y
DyLight 755
FAB3652Z
FITC
FAB3652F
HRP
FAB3652H
Janelia Fluor 525
FAB3652JF525
Janelia Fluor 549
FAB3652I
Janelia Fluor 646
FAB3652J
Janelia Fluor 669
FAB3652JF669
PE
FAB3652P
PerCP
FAB3652C
mFluor Violet 450 SE
FAB3652MFV450
mFluor Violet 500 SE
FAB3652MFV500
mFluor Violet 610 SE
FAB3652MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3652-SP
Carrier Free
MAB3652-500
Carrier Free
IC3652V-100UG
Carrier Free
IC3652U-100UG
Carrier Free
IC3652T-100UG
Carrier Free
IC3652S-100UG
Carrier Free
IC3652R-100UG
Carrier Free
IC3652N-100UG
Carrier Free
IC3652G-100UG
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CX3CL1/Fractalkine Antibody (81506) [Unconjugated] Summary

Immunogen
NS0-derived and E. coli-derived Recombinant Human CX3CL1/Fracktalkine
Gln25-Arg339
Accession # P78423
Specificity
Detects human CX3CL1/Fractalkine in ELISAs. In sandwich immunoassays, less than 10% cross-reactivity with recombinant mouse CXCL6 and less than 0.2% cross-reactivity with recombinant human (rh) CXCL6, rhCXCL9, and recombinant rat CX3CL1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CX3CL1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 1-5 ug/mL
Application Notes
ELISA Detection: Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog number BAM365)
Standard: Recombinant Human CX3CL1/Fractalkine Full Length (Catalog number 365-FR)
Publications
Read Publications using
MAB3652 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody (81506) [Unconjugated]

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CX3CL1/Fractalkine
Antibody (81506) [Unc ...
MAB3652
CX3CL1/Fractalkine Antibody (81506) [Unconjugated]
Species: Hu
Applications: B/N, CyTOF-ready, ELISA(Cap), Flow

Publications for CX3CL1/Fractalkine Antibody (MAB3652)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: ELISA Development, ELISA Development (Capture), Flow Cytometry, Functional Assay, Neutralization, Western Blot.


Filter By Application
ELISA Development
(3)
ELISA Development (Capture)
(2)
Flow Cytometry
(2)
Functional Assay
(1)
Neutralization
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(10)
All Species
Showing Publications 1 - 10 of 10.
Publications using MAB3652 Applications Species
J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy Nature Communications, 2021-02-17;12(1):1087. 2021-02-17 [PMID: 33597530] (ELISA Development, Human) ELISA Development Human
M Brown, LA Johnson, DA Leone, P Majek, K Vaahtomeri, D Senfter, N Bukosza, H Schachner, G Asfour, B Langer, R Hauschild, K Parapatics, YK Hong, KL Bennett, R Kain, M Detmar, M Sixt, DG Jackson, D Kerjaschki Lymphatic exosomes promote dendritic cell migration along guidance cues J. Cell Biol., 2018-04-12;0(0):. 2018-04-12 [PMID: 29650776] (Neutralization, Human) Neutralization Human
A Babendreye, L Molls, D Dreymuelle, S Uhlig, A Ludwig Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion Mediators Inflamm., 2017-03-26;2017(0):1515389. 2017-03-26 [PMID: 28522896] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis Arthritis Res. Ther, 2017-03-07;19(1):45. 2017-03-07 [PMID: 28270187] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Kassianos A, Wang X, Sampangi S, Afrin S, Wilkinson R, Healy H Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells. Kidney Int, 2015-01-14;87(6):1153-63. 2015-01-14 [PMID: 25587706] (Neutralization, Human) Neutralization Human
Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation. Mucosal Immunol, 2012-06-13;5(0):702. 2012-06-13 [PMID: 22692452] (Flow Cytometry, Human) Flow Cytometry Human
Nevo I, Sagi-Assif O, Meshel T, Ben-Baruch A, Johrer K, Greil R, Trejo LE, Kharenko O, Feinmesser M, Yron I, Witz IP The involvement of the fractalkine receptor in the transmigration of neuroblastoma cells through bone-marrow endothelial cells. Cancer Lett., 2008-09-07;273(1):127-39. 2008-09-07 [PMID: 18778890] (Flow Cytometry, Functional Assay, Human) Flow Cytometry, Functional Assay Human
Popovic M, Laumonnier Y, Burysek L, Syrovets T, Simmet T Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration. J. Leukoc. Biol., 2008-04-24;84(1):215-23. 2008-04-24 [PMID: 18436581] (Western Blot, Human) Western Blot Human
Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes. J. Immunol., 2007-06-15;178(12):8064-72. 2007-06-15 [PMID: 17548644] (ELISA Development, Human) ELISA Development Human
Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao JL, Maho M, Boissonnas A, Murphy PM, Debre P, Combadiere C Fractalkine mediates natural killer-dependent antitumor responses in vivo. Cancer Res., 2003-11-01;63(21):7468-74. 2003-11-01 [PMID: 14612547] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol CX3CL1
Uniprot