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CX3CL1/Fractalkine Antibody (51637) [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clone
51637
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC365U-100UG
Alexa Fluor 405
IC365V-100UG
Alexa Fluor 488
IC365G-100UG
Alexa Fluor 532
FAB365X
Alexa Fluor 594
IC365T-100UG
Alexa Fluor 647
IC365R-100UG
Alexa Fluor 700
IC365N-100UG
Alexa Fluor 750
IC365S-100UG
Allophycocyanin
IC365A
CoraFluor 1
FAB365CL1
DyLight 350
FAB365D
DyLight 405
FAB365E
DyLight 488
FAB365K
DyLight 550
FAB365L
DyLight 594
FAB365M
DyLight 650
FAB365W
DyLight 680
FAB365Y
DyLight 755
FAB365Z
FITC
FAB365F
HRP
FAB365H
Janelia Fluor 525
FAB365JF525
Janelia Fluor 549
FAB365I
Janelia Fluor 585
FAB365JF585
Janelia Fluor 635
FAB365JF635
Janelia Fluor 646
FAB365J
Janelia Fluor 669
FAB365JF669
PerCP
FAB365C
Phycoerythrin
IC365P
Unconjugated
MAB365-100
mFluor Violet 450 SE
FAB365MFV450
mFluor Violet 500 SE
FAB365MFV500
mFluor Violet 610 SE
FAB365MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB365-100
Carrier Free
IC365P
Carrier Free
IC365A
Carrier Free
MAB365-SP
Carrier Free
MAB365-500
Carrier Free
MAB365-100
Carrier Free
IC365V-100UG
Carrier Free
IC365U-100UG
Carrier Free
IC365T-100UG
Carrier Free
IC365S-100UG
Carrier Free
IC365R-100UG
Carrier Free
IC365N-100UG
Carrier Free
IC365G-100UG
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CX3CL1/Fractalkine Antibody (51637) [Biotin] Summary

Immunogen
NS0-derived and E.coli-derived Recombinant Human Fractalkine
Gln25-Arg339
Accession # P78423
Specificity
Detects human CX3CL1 in ELISAs and Western blots.  In ELISAs, this antibody shows approximately 8% cross-reactivity with recombinant mouse (rm) CXCL6 and less than 0.2% cross-reactivity with rhCXCL6, rhCXCL9, and rrCX3CL1.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CX3CL1
Purity Statement
Protein A or G purified from ascites
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Human CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # MAB3652)
ELISA Detection: Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog # BAM365)
Standard: Recombinant Human CX3CL1/Fractalkine Full Length (Catalog # 365-FR)
Readout System
Publications
Read Publications using
BAM365 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody (51637) [Biotin]

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

Fractalkine is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CX3CL1/Fractalkine
Antibody (51637) [Bio ...
BAM365
CX3CL1/Fractalkine Antibody (51637) [Biotin]
Species: Hu
Applications: WB, ELISA(Det)

Publications for CX3CL1/Fractalkine Antibody (BAM365)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA Development, ELISA Development (Detection).


Filter By Application
ELISA Development
(3)
ELISA Development (Detection)
(2)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 5 of 5.
Publications using BAM365 Applications Species
A Babendreye, L Molls, D Dreymuelle, S Uhlig, A Ludwig Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion Mediators Inflamm., 2017-03-26;2017(0):1515389. 2017-03-26 [PMID: 28522896] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis Arthritis Res. Ther, 2017-03-07;19(1):45. 2017-03-07 [PMID: 28270187] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Popovic M, Laumonnier Y, Burysek L, Syrovets T, Simmet T Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration. J. Leukoc. Biol., 2008-04-24;84(1):215-23. 2008-04-24 [PMID: 18436581] (ELISA Development, Human) ELISA Development Human
Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes. J. Immunol., 2007-06-15;178(12):8064-72. 2007-06-15 [PMID: 17548644] (ELISA Development, Human) ELISA Development Human
Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao JL, Maho M, Boissonnas A, Murphy PM, Debre P, Combadiere C Fractalkine mediates natural killer-dependent antitumor responses in vivo. Cancer Res., 2003-11-01;63(21):7468-74. 2003-11-01 [PMID: 14612547] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol CX3CL1
Uniprot