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CX3CL1/Fractalkine Antibody (126315) [Unconjugated]

 (12 Publications)
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Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571). The ND50 is typically 0.75‑3.5 µg/mL.
Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more
CX3CL1 expression in the brain during EAE progression. (A) Brain CX3CL1 expression of wild type mice with EAE as determined by quantitative real-time PCR. Gene levels were normalized to GAPDH levels and are displayed as ...read more
CX3CL1 antibody mediated blockade protects mice against EAE and its associated lymphocyte infiltration. Wild type mice were induced to develop EAE and starting at day 8 post induction given daily anti-CX3CL1 antibody or ...read more
CX3CL1 expression in the brain during EAE progression. (A) Brain CX3CL1 expression of wild type mice with EAE as determined by quantitative real-time PCR. Gene levels were normalized to GAPDH levels and are displayed as ...read more
CX3CL1 expression in the brain during EAE progression. (A) Brain CX3CL1 expression of wild type mice with EAE as determined by quantitative real-time PCR. Gene levels were normalized to GAPDH levels and are displayed as ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
126315
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB571U
Alexa Fluor 405
FAB571V
Alexa Fluor 532
FAB571X
Alexa Fluor 594
FAB571T
Alexa Fluor 647
FAB571R
Alexa Fluor 700
FAB571N
Alexa Fluor 750
FAB571S
Allophycocyanin
FAB571A-025
Biotin
FAB571B
CoraFluor 1
FAB571CL1
DyLight 350
FAB571D
DyLight 405
FAB571E
DyLight 488
FAB571K
DyLight 550
FAB571L
DyLight 594
FAB571M
DyLight 650
FAB571W
DyLight 680
FAB571Y
DyLight 755
FAB571Z
FITC
FAB571F
HRP
FAB571H
Janelia Fluor 525
FAB571JF525
Janelia Fluor 549
FAB571I
Janelia Fluor 585
FAB571JF585
Janelia Fluor 635
FAB571JF635
Janelia Fluor 646
FAB571J
Janelia Fluor 669
FAB571JF669
PerCP
FAB571C
Phycoerythrin
FAB571P-025
mFluor Violet 450 SE
FAB571MFV450
mFluor Violet 500 SE
FAB571MFV500
mFluor Violet 610 SE
FAB571MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB571-SP
Carrier Free
FAB571P-025
Carrier Free
FAB571A-025
Carrier Free
MAB571-500
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CX3CL1/Fractalkine Antibody (126315) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CX3CL1/Fractalkine, S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CX3CL1/Fractalkine, and E. coli-derived recombinant mouse CX3CL1/Fractalkine
Specificity
Detects mouse CX3CL1/Fractalkine in Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with the chemokine domain of recombinant human (rh) CX3CL1, 13% cross-reactivity with rhCX3CL1, and 50% cross-reactivity with recombinant rat CX3CL1.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CX3CL1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.75-3.5 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Mouse CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog number BAF472)
Standard: Recombinant Mouse CX3CL1/Fractalkine Full Length (Catalog number 472-FF)
Publications
Read Publications using
MAB571 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody (126315) [Unconjugated]

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CX3CL1/Fractalkine
Antibody (126315) [Un ...
MAB571
CX3CL1/Fractalkine Antibody (126315) [Unconjugated]
Species: Mu
Applications: WB, B/N, ELISA(Cap)

Publications for CX3CL1/Fractalkine Antibody (MAB571)(12)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, In Vivo, Neutralization.


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ICC
(1)
IHC
(1)
IHC-Fr
(1)
In Vivo
(1)
Neutralization
(4)
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Mouse
(6)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB571 Applications Species
Apoorvi Chaudhri, Xia Bu, Yunfei Wang, Michael Gomez, James A. Torchia, Ping Hua et al. The CX3CL1-CX3CR1 chemokine axis can contribute to tumor immune evasion and blockade with a novel CX3CR1 monoclonal antibody enhances response to anti-PD-1 immunotherapy Front Immunol 9/13/2023 [PMID: 37771579]
Xu Wang, Tao Zhou, Xingyi Chen, Yu Wang, Yushi Ding, Haoyang Tu et al. System analysis based on the cancer–immunity cycle identifies ZNF207 as a novel immunotherapy target for hepatocellular carcinoma Journal for ImmunoTherapy of Cancer 3/4/2022 [PMID: 35246476]
P Rappl, S Rösser, P Maul, R Bauer, A Huard, Y Schreiber, D Thomas, G Geisslinge, PJ Jakobsson, A Weigert, B Brüne, T Schmid Inhibition of mPGES-1 attenuates efficient resolution of acute inflammation by enhancing CX3CL1 expression Cell Death & Disease, 2021-02-02;12(2):135. 2021-02-02 [PMID: 33542207] (IHC, Neutralization, Mouse) IHC, Neutralization Mouse
Soulixay Senouthai, Junjie Wang, Dongdong Fu, Yanwu You Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/ beta -Catenin Pathway in an Acute Kidney Injury Mouse Model Inflammation 8/1/2019 [PMID: 30919150]
Mouton AJ, Ma Y, Rivera Gonzalez OJ et al. Fibroblast polarization over the myocardial infarction time continuum shifts roles from inflammation to angiogenesis. Basic Res. Cardiol. 1/11/2019 [PMID: 30635789]
M Mizoguchi, Y Ishida, M Nosaka, A Kimura, Y Kuninaka, T Yahata, S Nanjo, S Toujima, S Minami, K Ino, N Mukaida, T Kondo Prevention of lipopolysaccharide-induced preterm labor by the lack of CX3CL1-CX3CR1 interaction in mice PLoS ONE, 2018-11-06;13(11):e0207085. 2018-11-06 [PMID: 30399192] (Neutralization, Mouse) Neutralization Mouse
A Okuma, A Hanyu, S Watanabe, E Hara p16Ink4a and p21Cip1/Waf1 promote tumour growth by enhancing myeloid-derived suppressor cells chemotaxis Nat Commun, 2017-12-12;8(1):2050. 2017-12-12 [PMID: 29234059] (In Vivo, Mouse) In Vivo Mouse
Ukpong B. Eyo, Jiyun Peng, Madhuvika Murugan, Mingshu Mo, Almin Lalani, Ping Xie et al. Regulation of Physical Microglia-Neuron Interactions by Fractalkine Signaling after Status Epilepticus eNeuro 12/29/2016 [PMID: 28101527]
Bryan Simons PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen J. Immunol, 2016-11-28;0(0):. 2016-11-28 [PMID: 27895167] (Neutralization, Mouse) Neutralization Mouse
Manuel Tardáguila, Emilia Mira, Miguel A. García-Cabezas, Anna M. Feijoo, Miguel Quintela-Fandino, Iñigo Azcoitia et al. CX3CL1 Promotes Breast Cancer via Transactivation of the EGF Pathway Cancer Research 7/15/2013 [PMID: 23720051]
Show All 12 Publications.

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Bioinformatics

Gene Symbol CX3CL1