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CUGBP1/CELF1 Antibody (OTI5B8)

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Western Blot: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUGBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CUGBP1.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded prostate tissue using anti-CUGBP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CUGBP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CUGBP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-CUGBP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded Kidney tissue using anti-CUGBP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded liver tissue using anti-CUGBP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded pancreas tissue using anti-CUGBP1 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI5B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.99 mg/ml

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CUGBP1/CELF1 Antibody (OTI5B8) Summary

Immunogen
Full length human recombinant protein of human CUGBP1 (NP_006551) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CELF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.99 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CUGBP1/CELF1 Antibody (OTI5B8)

  • BRUNOL2
  • BRUNOL250 kDa nuclear polyadenylated RNA-binding protein
  • bruno-like 2
  • Bruno-like protein 2
  • CELF1
  • CUG RNA-binding protein
  • CUG triplet repeat, RNA binding protein 1
  • CUG triplet repeat, RNA-binding protein 1
  • CUG-BP- and ETR-3-like factor 1
  • CUGBP Elav-like family member 1
  • CUG-BP
  • CUGBP, Elav-like family member 1
  • CUGBP1
  • CUG-BP1
  • CUGBP1CUG triplet repeat RNA-binding protein 1
  • CUGBPCELF-1
  • D2Wsu101e
  • Deadenylation factor CUG-BP
  • EDEN-BP homolog
  • EDEN-BP
  • embryo deadenylation element binding protein
  • Embryo deadenylation element-binding protein homolog
  • HNAB50
  • NAB50
  • NAB50CUG-BP1
  • NAPOR
  • nuclear polyadenylated RNA-binding protein, 50-kD
  • RNA-binding protein BRUNOL-2

Background

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CELF1
Entrez