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CRY2 Antibody (OTI1H5)

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Western Blot: CRY2 Antibody (1H5) [NBP2-00749] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRY2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRY2.
Western Blot: CRY2 Antibody (1H5) [NBP2-00749] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRY2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: CRY2 Antibody (1H5) [NBP2-00749] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CRY2.
Immunohistochemistry-Paraffin: CRY2 Antibody (1H5) [NBP2-00749] - Staining of paraffin-embedded Human lymphoma tissue using anti-CRY2 mouse monoclonal antibody.
Flow Cytometry: CRY2 Antibody (1H5) [NBP2-00749] - Analysis of Jurkat cells, using anti-CRY2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: CRY2 Antibody (1H5) [NBP2-00749] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CRY2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CRY2 Antibody (1H5) [NBP2-00749] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CRY2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CRY2 Antibody (1H5) [NBP2-00749] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CRY2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CRY2 Antibody (1H5) [NBP2-00749] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CRY2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CRY2 Antibody (1H5) [NBP2-00749] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CRY2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CRY2 Antibody (1H5) [NBP2-00749] - Staining of paraffin-embedded Human colon tissue using anti-CRY2 mouse monoclonal antibody.
Flow Cytometry: CRY2 Antibody (1H5) [NBP2-00749] - Analysis of Hela cells, using anti-CRY2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.78 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70449AF350
Alexa Fluor 405
NBP2-70449AF405
Alexa Fluor 488
NBP2-70449AF488
Alexa Fluor 532
NBP2-70449AF532
Alexa Fluor 594
NBP2-70449AF594
Alexa Fluor 647
NBP2-70449AF647
Alexa Fluor 700
NBP2-70449AF700
Alexa Fluor 750
NBP2-70449AF750
Allophycocyanin
NBP2-70449APC
Allophycocyanin/Cy7
NBP2-70449APCCY7
Biotin
NBP2-70449B
CoraFluor 1
NBP2-70449CL1
DyLight 350
NBP2-70449UV
DyLight 405
NBP2-70449V
DyLight 488
NBP2-70449G
DyLight 550
NBP2-70449R
DyLight 594
NBP2-70449DL594
DyLight 650
NBP2-70449C
DyLight 680
NBP2-70449FR
DyLight 755
NBP2-70449IR
FITC
NBP2-70449F
HRP
NBP2-70449H
Janelia Fluor 525
NBP2-70449JF525
Janelia Fluor 549
NBP2-70449JF549
Janelia Fluor 585
NBP2-70449JF585
Janelia Fluor 635
NBP2-70449JF635
Janelia Fluor 646
NBP2-70449JF646
Janelia Fluor 669
NBP2-70449JF669
PE
NBP2-70449PE
PE/Atto594
NBP2-70449PEATT594
PE/Cy5.5
NBP2-70449PECY55
PE/Cy7
NBP2-70449PECY7
PerCP
NBP2-70449PCP
mFluor Violet 450 SE
NBP2-70449MFV450
mFluor Violet 500 SE
NBP2-70449MFV500
mFluor Violet 610 SE
NBP2-70449MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-70449
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CRY2 Antibody (OTI1H5) Summary

Immunogen
Full length human recombinant protein of human CRY2 (NP_0066940) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CRY2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
66.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.78 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CRY2 Antibody (OTI1H5)

  • CRY2
  • cryptochrome 2 (photolyase-like)
  • cryptochrome-2
  • FLJ10332
  • growth-inhibiting protein 37
  • HCRY2
  • KIAA0658
  • PHLL2

Background

Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK NPAS2-ARNTL E box-mediatedtranscription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has nophotolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. Mayinhibit CLOCK NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-00749
CRY2 Antibody (OTI1H5)
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Bioinformatics

Gene Symbol CRY2
Entrez