![Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6. Analysis of NR1D1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-Western-Blot-H00009572-M02-img0022.jpg "Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6. Analysis of NR1D1 expression in NIH/3T3.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, ChIP, KD |
| Clone | 4F6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | NR1D1 (AAH56148, 1 a.a. ~ 614 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQRHNEALNGLRQAPSSYPPTWPPGPAHHSCHQSNSNGHRLCPTHVYAAPEGKAPANSPRQGNSKNVLLACPMNMYPHGRSGRTVQEIWEDFSMSFTPAVREVVEFAKHIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFNVKDQTVMFLSRTTYSLQELGAMGMGDLLSAMFDFSEKLNSLALTEEELGLFTAVVLVSADRSGMENSASVEQLQETLLRALRALVLKNRPLETSRFTKLLLKLPDLRTLNNMHSEKLLSFRVDAQ |
| Specificity | NR1D1 (4F6) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NR1D1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | Protein A purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IF | Mouse | 02/26/2015 |
Summary
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reviewed by:
Verified Customer |
IHC-P | Human | 02/22/2015 |
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reviewed by:
Verified Customer |
IHC-P | Mouse | 02/22/2015 |
Summary
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reviewed by:
Hyelin Na |
IHC-P | Human | 09/26/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 02/26/2015 |
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| Application: | IF | |
| Species: | Mouse |
| Verified Customer 02/22/2015 |
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| Application: | IHC-P | |
| Species: | Human |
| Verified Customer 02/22/2015 |
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| Application: | IHC-P | |
| Species: | Mouse |
![Immunocytochemistry/Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-Immunocytochemistry-Immunofluorescence-H00009572-M02-img0016.jpg "Immunocytochemistry/Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Representative tissue microarray specimens stained for NR1D1. Immunoreactivity of representative tumor specimens is shown. Low and high NR1D1 expression groups are defined in methods. The low NR1D1 expression ranges from immunoreactive scores 2 to 6 (left (P2xI1), middle (P3xI1), and right (P3xI2)), and the high NR1D1 expression shows immunoreactive score 12 (all, P4xI3). Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-019-1197-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-Immunohistochemistry-Paraffin-H00009572-M02-img0024.jpg "Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Representative tissue microarray specimens stained for NR1D1. Immunoreactivity of representative tumor specimens is shown. Low and high NR1D1 expression groups are defined in methods. The low NR1D1 expression ranges from immunoreactive scores 2 to 6 (left (P2xI1), middle (P3xI1), and right (P3xI2)), and the high NR1D1 expression shows immunoreactive score 12 (all, P4xI3). Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-019-1197-x) licensed under a CC-BY license.")
![Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 expression in transfected 293T cell line by NR1D1 monoclonal antibody (M02), clone 4F6. Lane 1: NR1D1 transfected lysate (66.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-Western-Blot-H00009572-M02-img0002.jpg "Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 expression in transfected 293T cell line by NR1D1 monoclonal antibody (M02), clone 4F6. Lane 1: NR1D1 transfected lysate (66.8 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 over-expressed 293 cell line, cotransfected with NR1D1 Validated Chimera RNAi ( Cat # H00009572-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D1 monoclonal antibody (M02), clone 4F6 (Cat # H00009572-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-Western-Blot-H00009572-M02-img0019.jpg "Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 over-expressed 293 cell line, cotransfected with NR1D1 Validated Chimera RNAi ( Cat # H00009572-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D1 monoclonal antibody (M02), clone 4F6 (Cat # H00009572-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6 Analysis of NR1D1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-Western-Blot-H00009572-M02-img0021.jpg "Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6 Analysis of NR1D1 expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded mouse lung tissue using anti-NR1D1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-Immunohistochemistry-Paraffin-H00009572-M02-img0011.jpg "Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded mouse lung tissue using anti-NR1D1 antibody. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded human lung cancer tissue using anti-NR1D1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-Immunohistochemistry-Paraffin-H00009572-M02-img0012.jpg "Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded human lung cancer tissue using anti-NR1D1 antibody. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of Rev-erb A alpha in paraffin embedded mouse lung sections using anti-Rev-erb A alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-Immunohistochemistry-Paraffin-H00009572-M02-img0014.jpg "Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of Rev-erb A alpha in paraffin embedded mouse lung sections using anti-Rev-erb A alpha antibody. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Staining on human breast cancer tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-Immunohistochemistry-Paraffin-H00009572-M02-img0008.jpg "Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Staining on human breast cancer tissue. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-Immunohistochemistry-Paraffin-H00009572-M02-img0018.jpg "Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.")
![ELISA: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Detection limit for recombinant GST tagged NR1D1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Rev-erb-A-alpha-NR1D1-Antibody-4F6-ELISA-H00009572-M02-img0023.jpg "ELISA: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Detection limit for recombinant GST tagged NR1D1 is approximately 1ng/ml as a capture antibody.")
![Immunohistochemistry: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Representative tissue microarray specimens stained for NR1D1. Immunoreactivity of representative tumor specimens is shown. Low & high NR1D1 expression groups are defined in methods. The low NR1D1 expression ranges from immunoreactive scores 2 to 6 (left (P2xI1), middle (P3xI1), & right (P3xI2)), & the high NR1D1 expression shows immunoreactive score 12 (all, P4xI3). P, percentage of stained tumor cells; I, intensity of staining. Scale bar, 50 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31779659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00009572-m02_mouse-monoclonal-rev-erb-a-alpha-nr1d1-antibody-4f6-310202415304257.jpg "Immunohistochemistry: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Representative tissue microarray specimens stained for NR1D1. Immunoreactivity of representative tumor specimens is shown. Low & high NR1D1 expression groups are defined in methods. The low NR1D1 expression ranges from immunoreactive scores 2 to 6 (left (P2xI1), middle (P3xI1), & right (P3xI2)), & the high NR1D1 expression shows immunoreactive score 12 (all, P4xI3). P, percentage of stained tumor cells; I, intensity of staining. Scale bar, 50 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31779659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry CRY1 Antibody [Unconjugated]](https://images.novusbio.com/images2/CRY1_AF3764_Immunocytochemistry__Immunofluorescence_19530.jpg)
![Immunohistochemistry CRY1 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af3764_human-mouse-cry1-affinity-purified-polyclonal-ab-immunohistochemistry-16122024132946..jpg)
![Immunohistochemistry CRY1 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af3764_human-mouse-cry1-affinity-purified-polyclonal-ab-immunohistochemistry-16122024133047..jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")
