![Western Blot: COX IV [p Ser58] Antibody [NBP2-29532] - Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17k COXIV phosphorylated at Ser58 (Control). Immunolabeling is blocked by the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/COX-IV-[p-Ser58]-Antibody-Western-Blot-NBP2-29532-img0001.jpg "Western Blot: COX IV [p Ser58] Antibody [NBP2-29532] - Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17k COXIV phosphorylated at Ser58 (Control). Immunolabeling is blocked by the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH. Accession # P19783 |
| Modification | p Ser58 |
| Marker | Mitochondria Marker |
| Specificity | Specific for endogenous levels of the ~17 kDa COX4 protein phosphorylated at Ser58. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. |
| Preparation Method |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | COX4I1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
![COX4 [p Ser58] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "COX4 [p Ser58] Antibody")
Secondary Antibodies |
Isotype Controls |
Research Areas for COX4 Antibody (NBP2-29532)Find related products by research area.
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The Importance of the COX IV Antibody to Apoptosis Research COX IV isoform 1 is a nuclear-encoded polypeptide chain of the Cytochrome C Oxidase enzyme, located on the mitochondrial inner membrane. Owing to its widespread distribution in human and mammalian tissues, COX IV antibodies are widely used as loading ... Read full blog post. |
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