![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining in human skin and skeletal muscle tissues using anti-PTGS1 antibody. Corresponding PTGS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0012.jpg "Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining in human skin and skeletal muscle tissues using anti-PTGS1 antibody. Corresponding PTGS1 RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: MPDSFKVGSQEYSYEQFLFNTSMLVDYGVEALVDAFSRQIAGRIGGGRNMDHHILHVAVDVIRESREMRLQPFNEYRKRFGMKPYTSFQELVGEKEMAAELEELYGDIDALEFYPGLLLEKCHPNSIFGESMIEIGAPFSLKGLLGN |
| Predicted Species | Rat (90%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PTGS1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100. COX-1 antibody validated for Simple Western from a verified customer review. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
Simple Western | Mouse | 11/02/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for COX-1 Antibody (NBP1-85500)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 11/02/2017 |
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| Application: | Simple Western | |
| Species: | Mouse |
| Gene Symbol | PTGS1 |
![Western Blot: COX-1 Antibody [NBP1-85500] - Analysis in human cell line HELA.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NBP1-85500-img0005.jpg "Western Blot: COX-1 Antibody [NBP1-85500] - Analysis in human cell line HELA.")
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody [NBP1-85500] - Validation of gene expression changes from RNA-Seq experiments. b, d Cox1 and Krt5 expression in a control (b) and in the ShhCre;Ppargfl/fl mutant urothelium (d). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-12332-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85500-img0014.jpg "Immunocytochemistry/Immunofluorescence: COX-1 Antibody [NBP1-85500] - Validation of gene expression changes from RNA-Seq experiments. b, d Cox1 and Krt5 expression in a control (b) and in the ShhCre;Ppargfl/fl mutant urothelium (d). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-12332-0), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody [NBP1-85500] - Staining of human cell line BJ shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85500-img0009.jpg "Immunocytochemistry/Immunofluorescence: COX-1 Antibody [NBP1-85500] - Staining of human cell line BJ shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0010.jpg "Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining of human skeletal muscle shows low expression as expected.")
![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0011.jpg "Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining of human skin shows high expression.")
![Simple Western: COX-1 Antibody [NBP1-85500] - Lane view shows one specific signal (~70kDa) in lysates prepared from the mouse tail vein. Image from a verified customer review.](http://images.novusbio.com/fullsize/COX-1-Antibody-Simple-Western-NBP1-85500-img0013.jpg "Simple Western: COX-1 Antibody [NBP1-85500] - Lane view shows one specific signal (~70kDa) in lysates prepared from the mouse tail vein. Image from a verified customer review.")
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NBP1-85500] - Validation of gene expression changes from RNA-Seq experiments. a, c Expression of Cpt2 & Krt5 in the urothelium of Ppargfl/fl controls (a) & ShhCre;Ppargfl/fl mutants (c). b, d Cox1 & Krt5 expression in a control (b) & in the ShhCre;Ppargfl/fl mutant urothelium (d). e, g Sod1 & Krt5 expression in a Ppargfl/fl control urothelium (e) & in the urothelium of an ShhCre;Ppargfl/fl mutant (g). f, h Sod2 & Krt5 expression in the urothelium of a Ppargfl/fl control (f) & in a ShhCre;Ppargfl/fl mutant urothelium (h). i, l Uchl1 & Krt5 expression in the urothelium of a wild-type adult tongue (i) & in the urothelium of a ShhCre;Ppargfl/fl mutant (l). j, m Expression of Sprr1a & Krt5 in the urothelium of a control (j) & in a urothelium of a ShhCre;Ppargfl/fl mutant (m). k, n Cldn8 & Krt5 expression in a control urothelium (k) & in a ShhCre;Ppargfl/fl mutant (n). o, q)Krt6 & Krt5 expression in a control urothelium (o) & in a ShhCre;Ppargfl/fl mutant (q). p, r Uchl1 & Krt5 expression in a control urothelium (p) & in a ShhCre;Ppargfl/fl mutant (r) urothelium. Scale bars: 50 μm. Adult wild type, n = 6; adult mutant, n = 5 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31597917), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85500_rabbit-polyclonal-cox-1-antibody-310202416121933.jpg "Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NBP1-85500] - Validation of gene expression changes from RNA-Seq experiments. a, c Expression of Cpt2 & Krt5 in the urothelium of Ppargfl/fl controls (a) & ShhCre;Ppargfl/fl mutants (c). b, d Cox1 & Krt5 expression in a control (b) & in the ShhCre;Ppargfl/fl mutant urothelium (d). e, g Sod1 & Krt5 expression in a Ppargfl/fl control urothelium (e) & in the urothelium of an ShhCre;Ppargfl/fl mutant (g). f, h Sod2 & Krt5 expression in the urothelium of a Ppargfl/fl control (f) & in a ShhCre;Ppargfl/fl mutant urothelium (h). i, l Uchl1 & Krt5 expression in the urothelium of a wild-type adult tongue (i) & in the urothelium of a ShhCre;Ppargfl/fl mutant (l). j, m Expression of Sprr1a & Krt5 in the urothelium of a control (j) & in a urothelium of a ShhCre;Ppargfl/fl mutant (m). k, n Cldn8 & Krt5 expression in a control urothelium (k) & in a ShhCre;Ppargfl/fl mutant (n). o, q)Krt6 & Krt5 expression in a control urothelium (o) & in a ShhCre;Ppargfl/fl mutant (q). p, r Uchl1 & Krt5 expression in a control urothelium (p) & in a ShhCre;Ppargfl/fl mutant (r) urothelium. Scale bars: 50 μm. Adult wild type, n = 6; adult mutant, n = 5 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31597917), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
