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Porcine Corticotropin Releasing Factor ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma and other biological fluids
Standard Curve Range
12.35 - 1000 pg/mL
Sensitivity
5.09 pg/mL

Order Details

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Porcine Corticotropin Releasing Factor ELISA Kit (Colorimetric) Summary

Description
Assay time: 2 hours
Standard Curve Range
12.35 - 1000 pg/mL
Sensitivity
5.09 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Spike Recovery
78 - 100%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CRH

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Porcine Corticotropin Releasing Factor ELISA Kit (Colorimetric)

  • corticoliberin
  • corticotropin releasing hormone
  • Corticotropin-releasing hormone
  • CRFCorticotropin-releasing factor

Background

Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide derived from a 191-amino acid preprohormone. CRH issecreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in CRH hasbeen observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin dificiency hasmultiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to productionin the hypothalamus, CRH is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed inthe placenta. In the placenta CRH is a marker that determines the length of gestation and the timing of parturitionand delivery. A rapid increase in circulating levels of CRH occurs at the onset of parturition, suggesting that, inaddition to its metabolic functions, CRH may act as a trigger for parturition. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CRH