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Collagen IV Antibody [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, FeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP, IHC, KO, MS
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

Order Details

Collagen IV Antibody [DyLight 550] Summary

Immunogen
Collagen IV from human and bovine placenta (Uniprot: P02462)
Localization
Secreted and Cell surface (Potential).
Specificity
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL4A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Super-Resolution Microscopy
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Rat reactivity reported in scientific literature (PMID: 16774905)

Feline reactivity reported in scientific literature (PMID: 33091431).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Collagen IV Antibody [DyLight 550]

  • BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR
  • Collagen IV

Background

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COL4A1