Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free

Collagen III alpha 1, also referred to as collagen type III alpha 1 or COL3A1 for short, was first described in 1971 and is a member of the collagen superfamily and encoded COL3A1 gene (1, 2). In general, collagen III is an extracellular matrix protein that is synthesized as a preprocollagen followed by cleaving of the signal peptide to form the procollagen (1). The human COL3A1 gene is located on chromosome 2q32.2 and collagen III is synthesized as a homotrimer consisting of three identical alpha procollagen chains which are stabilized by disulfide bonds (1,2,3). Each alpha chain is 1466 amino acids (aa) in length with a theoretical molecular weight of 139 kDa for a single alpha chain (1). Structurally, each alpha chain is a left-handed helix which then join together to form a right-handed triple helix (1,2). C-terminal and N-terminal proteinases remove the globular ends of the procollagen to form the type III collagen (1).

Collagen III is a fibrillar collagen that constitutes 5-20% of all collagen in the body (1). It provides structural integrity and is found in many hallow organs and soft connective tissue including the vascular system, skin, lung, uterus, and intestine (1,2). Additionally, collagen III has be found to be associated with type I collagen in the same fibrils (1). Collagen III interacts with signaling integrins to carry out other key functions including cell adhesion, proliferation, and differentiation (1).

Mutations in the COL3A1 gene has been associated with a variety of human diseases, the most well-known being a group of connective tissue disorders termed Ehlers-Danlos Syndromes (1,2,4). Vascular Ehlers-Danlos Syndrome is a specific subtype that is considered the most severe and although the clinical manifestations vary, symptoms include thin skin and fragile blood vessels and can often result in both lung and heart complications (1,4). COL3A1 is also associated with glomerulopathies, or diseases of the glomeruli, which are characterized by an abundance of extracellular matrix (3). Collagenofibrotic glomerulopathy is one specific rare renal disease that is characterized by excessive levels of collagen III (3).

References

1. Kuivaniemi, H., & Tromp, G. (2019). Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. https://doi.org/10.1016/j.gene.2019.05.003

2. Ricard-Blum S. (2011). The collagen family. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a004978

3. Cohen A. H. (2012). Collagen Type III Glomerulopathies. Advances in chronic kidney disease. https://doi.org/10.1053/j.ackd.2012.02.017

4. Olson, S. L., Murray, M. L., & Skeik, N. (2019). A Novel Frameshift COL3A1 Variant in Vascular Ehlers-Danlos Syndrome. Annals of vascular surgery. https://doi.org/10.1016/j.avsg.2019.05.057

5. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.

6. Stephens LJ, Werkmeister JA, Tebb TA, Ramshaw JAM (1991) Identification of type III collagen from kangaroo skin. Das Leder 42:41-44.

7. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.

8. Werkmeister JA, Glattauer V, Tebb TA, Ramshaw JAM, Edwards GA, Robets G (1991) Structural stability of long-term implants of a collagen-based vascular prosthesis. J Long Term Eff Med Implants 1:107-119

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Rabbit.

We have publications tested in 8 applications: Block/Neutralize, ICC/IF, IF/IHC, IHC-Fr, IHC-P, Immunohistochemistry, WB, Western Blot.

Showing Publications 1 - 10 of 22. Show All 22 Publications. Collapse Publications.

Publications using NBP1-05119	Applications	Species
Liu C, Zhou D, Zhang Q et al. Transcription factor EB (TFEB) improves ventricular remodeling after myocardial infarction by inhibiting Wnt/?-catenin signaling pathway PeerJ 2023-08-18 [PMID: 37609444] (Western Blot, Block/Neutralize)	Western Blot, Block/Neutralize
Aslaner DM, Alghothani O, SaldaNa TA et al. E-cigarette vapor exposure in utero causes long-term pulmonary effects in offspring American journal of physiology. Lung cellular and molecular physiology 2022-10-11 [PMID: 36218276] (WB)	WB
Zhang D, Liu B, Jie X et al. Uncovering Bupi Yishen Formula Pharmacological Mechanisms Against Chronic Kidney Disease by Network Pharmacology and Experimental Validation Frontiers in Pharmacology 2021-11-15 [PMID: 34867380] (Western Blot, Block/Neutralize)	Western Blot, Block/Neutralize
Jiang X, Wojtkiewicz M, Patwardhan C et al. The effects of maturation and aging on the rotator cuff tendon-to-bone interface The FASEB Journal 2022-03-18 [PMID: 34822203] (Block/Neutralize)	Block/Neutralize
Yu L, Rao WT, Tang JD, Xing JF. Experimental investigation of early assessment of corpora cavernosa fibrosis with two-dimensional shear wave elastography Asian Journal of Andrology 2022-01-01 [PMID: 34494557] (Immunohistochemistry)	Immunohistochemistry
Li WH, Seo I, Kim B et al. Low-Level Red Plus Near Infrared Lights Combination Induces Expressions of Collagen and Elastin in Human Skin In Vitro International journal of cosmetic science 2021-02-16 [PMID: 33594706]
Boumil E, Castro N, Phillips A et al. USP10 targeted self-deliverable siRNA to prevent scarring in the cornea Nucleic Acids 2020-07-01 [PMID: 32829179] (IHC-Fr, Rabbit) Details: Citation using the Biotin format of this antibody.	IHC-Fr	Rabbit
Huynh T, Kim J, Dunlap G et al. In-vivo testing of an injectable matrix gel for the treatment of shoulder cuff muscle fatty degeneration Journal of Shoulder and Elbow Surgery 2020-06-01 [PMID: 32713662] (IF/IHC, IHC-Fr, Rabbit, Rat)	IF/IHC, IHC-Fr	Rabbit, Rat
Matsuo M Vitamin Cand eggshell membrane facilitate orthodontic tooth movement and induce histological changes in the periodontal tissue J Oral Biosci 2020-02-04 [PMID: 32032751] (ICC/IF, Rat)	ICC/IF	Rat
Cui H, He Y, Chen S et al. Macrophage-Derived miRNA-Containing Exosomes Induce Peritendinous Fibrosis after Tendon Injury through the miR-21-5p/Smad7 Pathway Mol Ther Nucleic Acids 2018-11-20 [PMID: 30594070] (WB, Mouse)	WB	Mouse
Kim SJ, Tatman PD, Song DH et al. Nanotopographic cues and stiffness control of tendon-derived stem cells from diverse conditions Int J Nanomedicine 2018-11-08 [PMID: 30510414] (ICC/IF, Rat)	ICC/IF	Rat
Learn G D, McClellan P E et al. Woven collagen biotextiles enable mechanically functional rotator cuff tendon regeneration during repair of segmental tendon defects in vivo. J Biomed Mater Res B Appl Biomater 2019-01-08 [PMID: 30485649] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Ramani Kritika, Tan Roderick J, Zhou Dong et al. IL-17 Receptor Signaling Negatively Regulates the Development of Tubulointerstitial Fibrosis in the Kidney. Mediators of Inflammation 2018-10-14 [PMID: 30405320] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Kim SJ, Song DH, Kim SJ. Characteristics of Tendon Derived Stem Cells According to Different Factors to Induce the Tendinopathy J. Cell. Physiol. 2018-01-17 [PMID: 29341108] (ICC/IF, Rat)	ICC/IF	Rat
Kim SJ, Song DH, Park JW et al. Effect of Bone Marrow Aspirate Concentrate-Platelet-Rich Plasma on Tendon-Derived Stem Cells and Rotator Cuff Tendon Tear Cell Transplant. 2017-05-09 [PMID: 28105983] (ICC/IF, Human)	ICC/IF	Human
Su L, Li X, Wu X et al. Simultaneous deactivation of FAK and Src improves the pathology of hypertrophic scar. Sci Rep 2016-05-16 [PMID: 27181267]
Zhou Y, Zhang J, Wu H et al. The differential effects of leukocyte-containing and pure platelet-rich plasma (PRP) on tendon stem/progenitor cells - implications of PRP application for the clinical treatment of tendon injuries. Stem Cell Res Ther 2015-09-16 [PMID: 26373929] (WB, ICC/IF, Rabbit)	WB, ICC/IF	Rabbit
Hong SJ, Jia SX, Xie P et al. Topically Delivered Adipose Derived Stem Cells Show an Activated-Fibroblast Phenotype and Enhance Granulation Tissue Formation in Skin Wounds. PLoS One 2013-01-01 [PMID: 23383253] (IHC-P, ICC/IF, Rabbit)	IHC-P, ICC/IF	Rabbit
Gokce A, Yilmaz I, Bircan R et al. Synergistic Effect of TGF-beta1 And BMP-7 on Chondrogenesis and Extracellular Matrix Synthesis: An In Vitro Study Open Orthop J 2012-01-01 [PMID: 23002411] (WB, Human)	WB	Human
Rortveit R, Lingaas F, Bonsdorff T et al. A canine autosomal recessive model of collagen type III glomerulopathy Lab Invest 2012-10-01 [PMID: 22890554] (IF/IHC, Canine)	IF/IHC	Canine
He Y, Yip SL, Cheung KK et al. The effect of monochromatic infrared energy on diabetic wound healing Int Wound J 2012-07-09 [PMID: 22776615] (IF/IHC, ICC/IF, Rat)	IF/IHC, ICC/IF	Rat
Ceylan S, Anik I, Koc K, Kokturk S, Ceylan S, Cine N, Savli H, Sirin G, Sam B, Gazioglu N. Microsurgical anatomy of membranous layers of the pituitary gland and the expression of extracellular matrix collagenous proteins. Acta Neurochir (Wien);153(12):2435-43; discussion 2443. 2011-12-01 [PMID: 21969225] (IF/IHC, Human)	IF/IHC	Human
Show All 22 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Paramananda Saikia	ICC	Rabbit	05/31/2018	View Summary Application Immunocytochemistry Sample Tested Eye lens tissue Species Rabbit Lot G0809

Array NBP1-05119

• Collagen III alpha 1/COL3A1 Antibodies
• Collagen III alpha 1/COL3A1 ELISA Kits
• Collagen III alpha 1/COL3A1 Proteins
• Collagen III alpha 1/COL3A1 Proteins and Enzyme