Porcine Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric) Summary
| Description |
Assay time: 3 hours |
| Standard Curve Range |
0.156 - 10 ng/mL |
| Sensitivity |
0.054 ng/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
%CV < 12 |
| Intra-Assay |
%CV < 10 |
| Spike Recovery |
79 - 94% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
F3 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated ready to use 96-well strip plate
- Standard Diluent
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
|
Alternate Names for Porcine Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)
Background
Tissue Factor encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Coagulation Factor III/Tissue Factor ELISA Kit (NBP3-42890). (Showing 1 - 1 of 1 FAQ).
-
Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
- Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.
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