RAW 264.7 mouse monocyte/macrophage cell line treated with LPS was stained with Goat Anti-Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3178P, ...read more
Detects mouse Coagulation Factor III in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Coagulation Factor III is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
F3
Purity Statement
Antigen Affinity-purified
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12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Coagulation Factor III/Tissue Factor Antibody [Phycoerythrin]
CD142 antigen
CD142
coagulation factor III (thromboplastin, tissue factor)
Coagulation Factor III
F3
FLJ17960
TF
TFA
Thromboplastin
Tissue Factor
Background
Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-294) (3, 4). The purified rmTF corresponds to the ectodomain (residues 29-251) and is potent in activating thermolysin-processed rmCoagulation Factor VII (R&D Systems, Catalog # 3305-SE) under the conditions described in the Activity Assay Protocol.
Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659.
Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496.
Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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FAQs for Coagulation Factor III/Tissue Factor Antibody (FAB3178P). (Showing 1 - 1 of 1 FAQs).
Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.
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