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CLOCK Antibody

Name: CLOCK Antibody
Brand: Novus Biologicals
SKU: AF4026
Price: 459 USD

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Western blot shows lysates of PC-3 human prostate cancer cell line and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Human CLOCK Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB
Clonality	Polyclonal
Host	Sheep
Conjugate	Unconjugated
Concentration	LYOPH

Order Details

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Catalog# & Formulation	Size	Price
Carrier Free AF4026-SP

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CLOCK Antibody Summary

Specificity	Detects human CLOCK in Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Sheep
Gene	CLOCK
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLOCK Antibody

BHLHE8
circadian locomoter output cycles protein kaput
Class E basic helix-loop-helix protein 8
clock (mouse) homolog
clock homolog (mouse)
CLOCK
EC 2.3.1.48
hCLOCK
KAT13D
KIAA0334bHLHe8circadian locomoter output cycles kaput protein

Background

CLOCK is an essential regulator of circadian rhythms. CLOCK is a basic helix-loop-helix-PAS transcription factor that heterodimerizes with BMAL1. Together these transcription factors bind to E box elements (CACGTG) within the promoter of circadian genes (period1 and 2) stimulating their transcription. BMAL dimerization is necessary for CLOCK’s nuclear translocation, E box dependent transcription, phosphorylation, and degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CLOCK Antibody (AF4026) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CLOCK Antibody (AF4026) (0)

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Secondary Antibodies

Isotype Controls

Additional CLOCK Products

Blogs on CLOCK

There are no specific blogs for CLOCK, but you can read our latest blog posts.

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Effects of SDF-1-CXCR4/CXCR7 pathway on MSC chemotaxis in vitro.(A) The chemotaxis in response to SDF-1 alpha (10 ng/ml for 12 h) was performed in the NP-MSCs and HP-MSCs treated with a neutralizing anti-CXCR4 antibody, an anti-CXCR7 antibody, and the respective isotype-matched control antibodies. *P<0.05, vs NP-MSCs; †P<0.05, vs the respective isotype-matched control antibodies. (B) NP-MSCs were transiently overexpressed with CXCR4 using pORF9-mCXCR4 vector or with CXCR7 using pORF9-mCXCR7 vector (n = 6). A negative control empty (pORF9-MCS) vector was used. (C) The transfected cells were subjected to chemotaxis in response to the indicated concentrations of SDF-1 alpha for 12 h. *P<0.05, vs the empty vector. (D and E) Western blot analysis (D) and FCM (E) were performed to determine the intracellular and extracellular expression of both CXCR4 and CXCR7 in the cells treated with or without SDF-1 alpha (50 ng/ml for 60 min). (F) The chemotaxis in response to SDF-1 alpha was performed in the cells treated with or without SDF-1 alpha . Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22511954), licensed under a CC-BY license. Not internally tested by R&D Systems.

Sheep IgG Isotype Control
5-001-A

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Bioinformatics

Gene Symbol	CLOCK
Uniprot	O15516()

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Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

CLOCK Antibody

Species Glossary

Images

Product Details

Order Details

CLOCK Antibody Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for CLOCK Antibody

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for CLOCK Antibody (AF4026) (0)

Reviews for CLOCK Antibody (AF4026) (0)

Product General Protocols

Video Protocols

FAQs for CLOCK Antibody (AF4026) (0)

Secondary Antibodies

Isotype Controls

Additional CLOCK Products

Blogs on CLOCK

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics