BMAL1 Antibody

BMAL1 (brain and muscle ARNT-like 1; ARNTL) is an essential core component in circadian clock machinery which is regulatory mechanism for circadian rhythms. Circadian clock genes include three period proteins (PER1, PER2 and PER3), two cryptochromes (CRY1 and CRY2), CLOCK, NPAS2 and BMAL proteins, wherein BMAL1 has a potential to heterodimerize with CLOCK or NPAS2 genes; and this neocomplex drives transcription from E-box elements (5'-CACGTG-3') found in circadian-responsive genes's promoters. PER/CRY proteins negatively regulate CLOCK/BMAL1 dimer-mediated transcription, thereby forming the feedback loop that regulates the timing of clock gene transcription. BMAL1 also associates with GNB2L1/RACK1 and PRKCA in a nuclear complex, whertein GNB2L1 and PRKCA are recruited to the complex in a circadian manner. BMAL1 undergoes acetylation (Lys-538), phosphorylation and sumoylation (Lys-259) upon dimerization with CLOCK and acetylation facilitates CRY1-mediated repression. CLOCK-BMAL1 double mutations within PAS domains leads to syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity. Clock genes functions primarily as tumor suppressors and their abbarant expression is observed in malignant pleural mesothelioma, colorectal and breast cancer.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 10 applications: B/N, Chemotaxis, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, KD, WB.

Showing Publications 1 - 10 of 66. Show All 66 Publications. Collapse Publications.

Publications using NB100-2288	Applications	Species
Shearn, CT;Anderson, AL;Devereaux, MW;El Kasmi, KC;Orlicky, DJ;Sokol, RJ; Expression of circadian regulatory genes is dysregulated by increased cytokine production in mice subjected to concomitant intestinal injury and parenteral nutrition PloS one 2023-08-30 [PMID: 37647292] (WB)	WB
Halawani, D;Wang, Y;Ramakrishnan, A;Estill, M;He, X;Shen, L;Friedel, RH;Zou, H; Circadian clock regulator Bmal1 gates axon regeneration via Tet3 epigenetics in mouse sensory neurons Nature communications 2023-08-24 [PMID: 37620297]
Fu J, Fan Z, He L et al. Circadian clock disruption in autoimmune thyroiditis European Thyroid Journal 2023-10-01 [PMID: 37548297] (IHC, ICC/IF)	IHC, ICC/IF
Gobbo F, Zingariello M, Verachi P et al. GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosis bioRxiv 2023-09-19 [PMID: 37425686]
Kinane C, Calligaro H, Jandot A et al. Dopamine modulates the retinal clock through melanopsin-dependent regulation of cholinergic waves during development BMC biology 2023-06-26 [PMID: 37365544] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Sheehan PW, Nadarajah CJ, Kanan MF et al. An astrocyte BMAL1-BAG3 axis protects against alpha-synuclein and tau pathology Neuron 2023-06-06 [PMID: 37315555] (IHC, WB, KD, Mouse)	IHC, WB, KD	Mouse
Schwartz PB, Nukaya M, Berres ME et al. The circadian clock is disrupted in pancreatic cancer PLOS Genetics 2023-06-01 [PMID: 37262074] (WB)	WB
Mendoza-Viveros L, Marmolejo-Gutierrez C, Cid-Castro C et al. Astrocytic circadian clock control of energy expenditure by transcriptional stress responses in the ventromedial hypothalamus Glia 2023-03-15 [PMID: 36919670]
Schwartz PB, Walcheck MT, Nukaya M et al. Chronic jetlag accelerates pancreatic neoplasia in conditional Kras-mutant mice Chronobiology international 2023-04-01 [PMID: 36912021] (Mouse)		Mouse
Gonzalez JC, Lee H, Vincent AM et al. Circadian regulation of dentate gyrus excitability mediated by G-protein signaling Cell reports 2023-02-06 [PMID: 36749664] (IHC, Mouse)	IHC	Mouse
Zhang C, Chen L, Sun L et al. BMAL1 collaborates with CLOCK to directly promote DNA double-strand break repair and tumor chemoresistance Oncogene 2023-02-02 [PMID: 36725890] (WB, ICC/IF)	WB, ICC/IF
Chen YT, Huang PY, Chai CY et al. Early detection of the initial stages of LED light-triggered non-alcoholic fatty liver disease by wax physisorption kinetics-Fourier transform infrared imaging The Analyst 2023-01-31 [PMID: 36621928] (IHC-P, Mouse) Details: 1:400 IHC-P dilution	IHC-P	Mouse
Dean T, Koffi AV, Goldstein E et al. Endogenous Circadian Clock Machinery in Cortical NG2-Glia Regulates Cellular Proliferation eNeuro 2022-09-19 [PMID: 36123116] (ICC/IF)	ICC/IF
Jang D, Yang B, You M et al. Fluoxetine decreases phagocytic function via REV-ERB alpha in microglia Research Square 2022-07-05 [PMID: 36048349]
Petrus P, Smith JG, Koronowski KB et al. The central clock suffices to drive the majority of circulatory metabolic rhythms Science Advances 2022-07-01 [PMID: 35767612] (IHC)	IHC
Singla R, Mishra A, Lin H et al. Haploinsufficiency of a Circadian Clock Gene Bmal1 (Arntl or Mop3) Causes Brain-Wide mTOR Hyperactivation and Autism-like Behavioral Phenotypes in Mice International journal of molecular sciences 2022-06-05 [PMID: 35682995] (WB, Mouse)	WB	Mouse
Vanmunster M, Rojo Garcia AV, Pacolet A et al. Mechanosensors control skeletal muscle mass, molecular clocks, and metabolism Cellular and molecular life sciences : CMLS 2022-05-27 [PMID: 35622133]
Becker-Krail DD, Ketchesin KD, Burns JN et al. Astrocyte Molecular Clock Function in the Nucleus Accumbens Is Important for Reward-Related Behavior Biological Psychiatry 2022-07-01 [PMID: 35461698] (B/N)	B/N
Hung CJ, Yamanaka A, Ono D. Conditional Knockout of Bmal1 in Corticotropin-Releasing Factor Neurons Does Not Alter Sleep-Wake Rhythm in Mice Frontiers in Neuroscience 2022-02-18 [PMID: 35250437] (IHC)	IHC
Ye P, Li W, Huang X et al. BMAL1 regulates mitochondrial homeostasis in renal ischaemia-reperfusion injury by mediating the SIRT1/PGC-1alpha axis Journal of cellular and molecular medicine 2022-02-17 [PMID: 35174626] (WB, Rat, Human)	WB	Rat, Human
McKee CA, Lee J, Cai Y et al. Astrocytes deficient in circadian clock gene Bmal1 show enhanced activation responses to amyloid-beta pathology without changing plaque burden Scientific reports 2022-02-02 [PMID: 35110643] (IF/IHC, Mouse)	IF/IHC	Mouse
de Zavalia N, Schoettner K, Goldsmith JA Et al. Bmal1 in the striatum influences alcohol intake in a sexually dimorphic manner Communications biology 2021-10-26 [PMID: 34702951] (WB, Mouse)	WB	Mouse
Gegnaw ST, Sandu C, Mendoza J Et al. Dark-adapted light response in mice is regulated by a circadian clock located in rod photoreceptors Experimental eye research 2021-10-22 [PMID: 34695438] (IF/IHC, Mouse)	IF/IHC	Mouse
Ferraro S, de Zavalia N, Belforte N, Amir S. In utero Exposure to Valproic-Acid Alters Circadian Organisation and Clock-Gene Expression: Implications for Autism Spectrum Disorders Frontiers in Behavioral Neuroscience 2021-09-28 [PMID: 34650409] (IHC, ICC/IF)	IHC, ICC/IF
Trebucq L, Cardama G, Lorenzano Menna P Et Al. Timing of Novel Drug 1A-116 to Circadian Rhythms Improves Therapeutic Effects against Glioblastoma Pharmaceutics 2021-07-16 [PMID: 34371781] (WB)	WB
Huang W, Zheng X, Yang M et al. PER2-mediated ameloblast differentiation via PPAR gamma/AKT1/beta-catenin axis International journal of oral science 2021-05-19 [PMID: 34011974]
Hosono T, Ono M, Daikoku T et al. Time-Restricted Feeding Regulates Circadian Rhythm of Murine Uterine Clock Current Developments in Nutrition 2021-05-01 [PMID: 33981944] (ICC/IF, IHC)	ICC/IF, IHC
Mosna K, Janega P, Sedlak J, Babal P. Complex changes of circadian proteins expression in inflammatory bowel disease Bratislava Medical Journal 2021-03-17 [PMID: 33729814] (IHC)	IHC
Kim H, Wei J, Song Z et al. Regulation of Hepatic Circadian Metabolism by the E3 ubiquitin ligase HRD1-controlled CREBH/PPARalpha Transcriptional Program Molecular metabolism 2021-02-13 [PMID: 33592335] (WB, Mouse)	WB	Mouse
Kim B, Kim J, Chun M, et al. Multiplexed CRISPR-Cas9 system in a single adeno-associated virus to simultaneously knock out redundant clock genes Scientific reports 2021-01-28 [PMID: 33510438] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Figueiro MG, Goo YH, Hogan R et al. Light-Dark Patterns Mirroring Shift Work Accelerate Atherosclerosis and Promote Vulnerable Lesion Phenotypes Journal of the American Heart Association 2021-01-19 [PMID: 33401929] (IHC-Fr)	IHC-Fr
Wang XL, Wolff SEC, Korpel N et al. Deficiency of the Circadian Clock Gene Bmal1 Reduces Microglial Immunometabolism Frontiers in immunology 2020-12-08 [PMID: 33363534] (WB, Mouse)	WB	Mouse
Greco CM, Cervantes M, Fustin JM et al. S-adenosyl-l-homocysteine hydrolase links methionine metabolism to the circadian clock and chromatin remodeling Science advances 2020-12-01 [PMID: 33328229] (ICC/IF, Mouse)	ICC/IF	Mouse
Sominsky L, Dangel T, Malik S et al. Microglial ablation in rats disrupts the circadian system Nat Commun 2020-11-13 [PMID: 33200466] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Yoo ID, Park MW, Cha HW et al. Elevated CLOCK and BMAL1 Contribute to the Impairment of Aerobic Glycolysis from Astrocytes in Alzheimer's Disease International Journal of Molecular Sciences 2020-10-23 [PMID: 33114015] (ICC/IF, IHC-P)	ICC/IF, IHC-P
Pulido RS, Munji RN, Chan TC et al. Neuronal Activity Regulates Blood-Brain Barrier Efflux Transport through Endothelial Circadian Genes Neuron 2020-12-01 [PMID: 32979312] (FLOW)	FLOW
Said R, Lobanova L, Papagerakis S, Papagerakis P. Calcium Sets the Clock in Ameloblasts Frontiers in Physiology 2020-07-31 [PMID: 32848861] (IHC)	IHC
Kim ER, Xu Y, Cassidy RM et al. Paraventricular hypothalamus mediates diurnal rhythm of metabolism Nat Commun 2020-07-30 [PMID: 32732906] (IF/IHC, Mouse)	IF/IHC	Mouse
Dan H, Ruan T, Sampogna RV Circadian Clock Regulation of Developmental Time in the Kidney Cell Rep 2020-05-19 [PMID: 32433970] (ICC/IF, Mouse)	ICC/IF	Mouse
Hasegawa S, Fukushima H, Hosoda H et al. Hippocampal clock regulates memory retrieval via Dopamine and PKA-induced GluA1 phosphorylation Nat Commun 2019-12-18 [PMID: 31852900] (WB, Mouse)	WB	Mouse
Yuan P, Yang T, Mu J et al. Circadian clock gene NPAS2 promotes reprogramming of glucose metabolism in hepatocellular carcinoma cells Cancer Lett. 2019-11-22 [PMID: 31765736] (Chemotaxis, Human)	Chemotaxis	Human
Wang C, Holtzman DM. The Bidirectional Relationship between the Circadian Clock and Alzheimer's disease Thesis 2022-01-01 [PMID: 31408876] (IHC-P, Mouse) Details: 1:2000 dilution IHC-P	IHC-P	Mouse
Hannibal J, Norn THB, Georg B et al. Spatiotemporal expression pattern of PERIOD1 and PERIOD 2 in the mouse SCN is dependent on VIP receptor2 signaling Eur. J. Neurosci. 2019-06-18 [PMID: 31211910]
Luo Q, Xiao Y, Alex A et al. The Diurnal Rhythm of Insulin Receptor Substrate-1 (IRS-1) and Kir4.1 in Diabetes: Implications for a Clock Gene Bmal1 Invest. Ophthalmol. Vis. Sci. 2019-05-01 [PMID: 31042800] (WB, Rat, Human)	WB	Rat, Human
Cedernaes J, Huang W, Ramsey KM et al. Transcriptional Basis for Rhythmic Control of Hunger and Metabolism within the AgRP Neuron Cell Metab. 2019-02-18 [PMID: 30827863] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhu M, Tang H, Tang X et al. BMAL1 suppresses ROS-induced endothelial-to-mesenchymal transition and atherosclerosis plaque progression via BMP signaling. Am J Transl Res. 2018-10-15 [PMID: 30416657] (WB, Human)	WB	Human
Lananna B V, Nadarajah C J et al. Cell-Autonomous Regulation of Astrocyte Activation by the Circadian Clock Protein BMAL1. Cell Rep 2018-02-10 [PMID: 30282019] (IF/IHC, Mouse)	IF/IHC	Mouse
Das V, Kc R, Li X, Varma D. Pharmacological targeting of the mammalian clock reveals a novel analgesic for osteoarthritis-induced pain. Gene. 2018-02-20 [PMID: 29474860] (Mouse)		Mouse
Kress GJ, Liao F, Dimitry J et al. Regulation of amyloid-B dynamics and pathology by the circadian clock. J. Exp. Med. 2018-04-02 [PMID: 29382695] (ICC/IF, Mouse)	ICC/IF	Mouse
Bostrom J, Sramkova Z, Sala?ova A et al. Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells. PLoS One 2017-12-11 [PMID: 29228002] (ICC/IF, Human)	ICC/IF	Human
Lai B, Zou J, Lin Z et al. Haploinsufficiency of hnRNP U Changes Activity Pattern and Metabolic Rhythms Am. J. Pathol. 2017-11-09 [PMID: 29128567] (Mouse)		Mouse
Zagni C, Almeida L O et al. PTEN Mediates Activation of Core Clock Protein BMAL1 and Accumulation of Epidermal Stem Cells. Stem Cell Reports 2017-11-07 [PMID: 28602615] (IF/IHC, Human)	IF/IHC	Human
Frederick A, Goldsmith J, de Zavalia N, Amir S. Mapping the co-localization of the circadian proteins PER2 and BMAL1 with enkephalin and substance P throughout the rodent forebrain. PLoS ONE. 2017-04-19 [PMID: 28423013] (ICC/IF, Rat)	ICC/IF	Rat
Dumbell R, Leliavski A, Matveeva O et al. Dissociation of Molecular and Endocrine Circadian Rhythms in Male Mice Lacking Bmal1 in the Adrenal Cortex. Endocrinology 2016-11-01 [PMID: 27690690] (Mouse)		Mouse
Matsumoto CS, Almeida LO, Guimaraes DM et al. PI3K-PTEN dysregulation leads to mTOR-driven upregulation of the core clock gene BMAL1 in normal and malignant epithelial cells. Oncotarget. 2016-07-05 [PMID: 27285754] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Perelis M, Marcheva B, Ramsey KM et al. Pancreatic B cell enhancers regulate rhythmic transcription of genes controlling insulin secretion. Science. 2015-11-06 [PMID: 26542580] (IHC-P, Mouse)	IHC-P	Mouse
Izumo M, Pejchal M, Schook AC et al. Differential effects of light and feeding on circadian organization of peripheral clocks in a forebrain Bmal1 mutant Elife. 2014-12-18 [PMID: 25525750] (WB, Mouse)	WB	Mouse
Leliavski A, Shostak A, Husse J, Oster H. Impaired glucocorticoid production and response to stress in Arntl-deficient male mice. Endocrinology. 2014-01-01 [PMID: 24189141] (IHC-P)	IHC-P
Obi-Ioka Y, Ushijima K, Kusama M et al. Involvement of Wee1 in the circadian rhythm dependent intestinal damage induced by docetaxel. J Pharmacol Exp Ther 2013-07-26 [PMID: 23892568] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Kim B, Kim J, Chun M et al. Single adeno-associated virus-based multiplexed CRISPR-Cas9 system to nullify core components of the mammalian molecular clock bioRxiv 2020-07-04 (WB, Mouse)	WB	Mouse
Petkau N TIP60 acetylation of BMAL1 links positive and negative arms of the molecular circadian clock Thesis (Chemotaxis, WB, Mouse)	Chemotaxis, WB	Mouse
Lauzier A, Charbonneau M, Harper K et al. Formation of invadopodia-like structures by synovial cells promotes cartilage breakdown in collagen-induced arthritis: Involvement of the protein tyrosine kinase Src. Arthritis Rheum;63(6):1591-1602. 2011 Jun. [PMID: 21337539] (ICC/IF, Rat)	ICC/IF	Rat
vorgelegt v, Wen W Expression and function of blood receptors ROR?, REVERBA and BMAL1 in human colorectal cancer Thesis 2022-01-01 (IHC, Mouse)	IHC	Mouse
Chu A, Zhu L, Blum ID et al. Global but not gonadotrope-specific disruption of bmal1 abolishes the luteinizing hormone surge without affecting ovulation. Endocrinology 2013-08-01 [PMID: 23736292] (ICC/IF, IHC-Fr, Mouse)	ICC/IF, IHC-Fr	Mouse
Hayashida S, Kuramoto Y, Koyanagi S et al. Proxisome proliferator-activated receptor-alpha mediates high-fat, diet-enhanced daily oscillation of plasminogen activator inhibitor-1 activity in mice. Chronobiol Int 2010-10-01 [PMID: 20969520] (Chemotaxis, WB, Mouse)	Chemotaxis, WB	Mouse
vorgelegt v, Wen W Expression and function of blood receptors ROR alpha , REVERBA and BMAL1 in human colorectal cancer Thesis 2022-01-01 (IF/IHC, Mouse)	IF/IHC	Mouse
Show All 66 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	3 reviewed by: Deborah Lutterschmidt	IHC-Fr	Hyla cinerea	10/20/2023	View Summary Application Immunohistochemistry-Frozen Sample Tested Brain Species Hyla cinerea Lot D146902 Comments Comments ***Novus Innovators Program - new species or application used on a primary antibody.***
Enlarge	5 reviewed by: Verified Customer	IF	Mouse	06/29/2015	View Summary Application Immunofluorescence Sample Tested mouse brain Species Mouse

Array NB100-2288

• BMAL1 Antibodies
• BMAL1 ELISA Kits
• BMAL1 Lysates
• BMAL1 Proteins