Novus Biologicals products are now on bio-techne.com

HSD17B4 Antibody

Images

 
Independent Antibodies: Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85296] - Staining of human liver, lung, lymph node and testis using Anti-HSD17B4 antibody NBP1-85296 (A) shows similar protein ...read more
Western Blot: HSD17B4 Antibody [NBP1-85296] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody [NBP1-85296] - Staining of human cell line A-431 shows localization to peroxisomes. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85296] - Staining of human lung.
Western Blot: HSD17B4 Antibody [NBP1-85296] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85296] - Staining of human liver.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85296] - Staining of human testis.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85296] - Staining of human lymph node.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

HSD17B4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ESCEENGGLFEVGAGWIGKLRWERTLGAIVRQKNHPMTPEAVKANWKKICDFENASKPQSIQESTGSIIEVLSK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSD17B4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
HSD17B4 Protein (NBP1-85296PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-85296 in the following applications:

Publications
Read Publications using
NBP1-85296 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HSD17B4 Antibody

  • 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase
  • 7-alpha
  • D-bifunctional protein
  • EDH17B4
  • hydroxysteroid (17-beta) dehydrogenase 4
  • MPF-2
  • peroxisomal multifunctional enzyme type 2,17-beta-HSD IV
  • peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV
  • peroxisomal
  • short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Background

The protein encoded by the HSD17B4 gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathwayfor fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chainand 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidationactivity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present onchromosome 8. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1513
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NB100-62346
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
NBP2-34294
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
AF2307
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), WB
NB600-717
Species: Bv, Ca, Ch, Fe, Gp, Hu, Ma, Mu, Po, Rb, Rt, Sh
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
NBP3-04784
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-1793
Species: Hu, Pm, Mu, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
NBP1-31348
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF2009
Species: Hu
Applications: ICC, IHC
MAB4447
Species: Hu
Applications: IHC, WB
NBP1-88027
Species: Eq, Hu, Mu, Rt
Applications: IHC, IHC-P, Simple Western, WB
AF7895
Species: Hu
Applications: IHC, WB
NBP2-20383
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
DY1707
Species: Hu
Applications: ELISA
NBP2-02623
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
DSHBG0B
Species: Hu
Applications: ELISA
NBP2-13304
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-2288
Species: Am, Hu, Mu, Pm, Rt
Applications: ChIP, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB

Publications for HSD17B4 Antibody (NBP1-85296)(3)

Review for HSD17B4 Antibody (NBP1-85296) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human and human embryonic kidney 293 cells.

Reviews using NBP1-85296:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human and human embryonic kidney 293 cells
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot HSD17B4 NBP1-85296
Enlarge
4
reviewed by:
Zhihui Zhu
WB Human and human embryonic kidney 293 cells 12/12/2018
View

Summary

ApplicationWestern Blot
Sample Tested20 micro gram,20 ug whole cell lysate
SpeciesHuman and human embryonic kidney 293 cells
LotR09826

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSD17B4 Antibody (NBP1-85296) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Zhihui Zhu
12/12/2018
Application: WB
Species: Human and human embryonic kidney 293 cells

Bioinformatics

Gene Symbol HSD17B4