Claudin-18 Antibody - BSA Free Summary
| Immunogen |
This antibody was developed against a recombinant protein corresponding to amino acids: MMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
CLDN18 |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunohistochemistry 1:500 - 1:1000
- Immunohistochemistry-Paraffin 1:500 - 1:1000
|
| Application Notes |
For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
| Control Peptide |
|
| Publications |
|
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (80%)
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
PBS (pH 7.2) and 40% Glycerol |
| Preservative |
0.02% Sodium Azide |
| Purity |
Immunogen affinity purified |
Alternate Names for Claudin-18 Antibody - BSA Free
Background
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu
Applications: CyTOF-ready, Flow
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: CyTOF-ready, Flow
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Pm, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: CyTOF-ready, Flow, ICC
Species: Hu
Applications: Flow, ICC/IF, IF, IHC, IHC-P, IP, WB
Species: Bv(-), Ch, Fe, Hu, Ma, Pm, Mu, Po, Rb, Rt
Applications: DB, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: IHC, WB
Species: Bv, Eq, Hu, Mu, Ma-Op, Pm, Rt
Applications: IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu, Mu, Po, Rt
Applications: ICC/IF
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, KD, WB
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Publications for Claudin-18 Antibody (NBP2-32002)(1)
Showing Publication 1 -
1 of 1.
| Publication using NBP2-32002 |
Applications |
Species |
| Jasani B, Schildhaus H, Dodson A et al. 7P Global ring study determining reproducibility & comparability of CLDN18 testing assays in gastric cancer ESMO Open 2023-02-01 |
|
|
Reviews for Claudin-18 Antibody (NBP2-32002) (0)
There are no reviews for Claudin-18 Antibody (NBP2-32002).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Claudin-18 Antibody (NBP2-32002) (0)
Secondary Antibodies
| |
Isotype Controls
|
Additional Claudin-18 Products
Research Areas for Claudin-18 Antibody (NBP2-32002)
Find related products by research area.
|
Blogs on Claudin-18
![Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - analysis in human stomach and liver tissues. Corresponding CLDN18 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Claudin-18-Antibody-Immunohistochemistry-Paraffin-NBP2-32002-img0004.jpg "Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - analysis in human stomach and liver tissues. Corresponding CLDN18 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human stomach shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Claudin-18-Antibody-Immunohistochemistry-Paraffin-NBP2-32002-img0009.jpg "Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human stomach shows strong membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Claudin-18-Antibody-Immunohistochemistry-Paraffin-NBP2-32002-img0003.jpg "Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human liver shows low expression as expected.")
![Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human tonsil shows weak to moderate membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Claudin-18-Antibody-Immunohistochemistry-Paraffin-NBP2-32002-img0005.jpg "Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human tonsil shows weak to moderate membranous positivity in squamous epithelial cells.")
![Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human stomach cancer shows moderate to strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/Claudin-18-Antibody-Immunohistochemistry-Paraffin-NBP2-32002-img0006.jpg "Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human stomach cancer shows moderate to strong membranous positivity in tumor cells.")
![Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human lung shows moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/Claudin-18-Antibody-Immunohistochemistry-Paraffin-NBP2-32002-img0007.jpg "Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human lung shows moderate membranous positivity in pneumocytes.")
![Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human pancreatic cancer shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/Claudin-18-Antibody-Immunohistochemistry-Paraffin-NBP2-32002-img0008.jpg "Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human pancreatic cancer shows moderate membranous positivity in tumor cells.")
![Flow Cytometry Claudin-4 Antibody (382321) [Unconjugated]](https://images.novusbio.com/images2/Claudin4_MAB4219_Flow_Cytometry_21352.jpg)
![Immunocytochemistry Claudin-6 Antibody (342927) [Unconjugated]](https://images.novusbio.com/images2/Claudin6_MAB3656_Immunocytochemistry_15896.jpg)
![Flow Cytometry Claudin-6 Antibody (342927) [Unconjugated]](https://images.novusbio.com/images2/Claudin6_MAB3656_Flow_Cytometry_22547.jpg)
![Immunohistochemistry Reg4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Reg4_AF1379_Immunohistochemistry_7311.jpg)
![Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neprilysin_AF1126_Immunohistochemistry_6679.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-8220249505976.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-44202415433122.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")