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Claudin-12 Antibody (393314)

Images

 
Caco‑2 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human Claudin‑12 Monoclonal Antibody (Catalog # MAB4284, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), ...read more
Claudin-12 was detected in immersion fixed NS0 mouse myeloma cell line transfected with human Claudin 12 using 10 µg/mL Mouse Anti-Human Claudin-12 Monoclonal Antibody (Catalog # MAB4284) for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
393314
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Claudin-12 Antibody (393314) Summary

Immunogen
HEK293 human embryonic kidney cell line transfected with human Claudin-12
Met1-Thr244
Accession # NP_036261
Specificity
Detects human Claudin-12. Stains human Claudin-12 transfectants but not irrelevant transfectants.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CLDN12
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claudin-12 Antibody (393314)

  • claudin 12
  • Claudin12
  • Claudin-12
  • CLDN12

Background

Claudin‑12 is a 244 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin‑12 is expressed on intestinal, bladder, and blood‑brain barrier epithelial cells. Human Claudin‑12 shares 92% aa sequence identity with mouse and rat Claudin‑12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CLDN12
Uniprot