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Calcitonin R Antibody (462802) [Unconjugated]

 (6 Publications)
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MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by ...read more
Genetic Strategies: Calcitonin R knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody ...read more
Effects of AA and DHA on actin ring formation and CTR expression.Osteoclast differentiation was stimulated in CD14+ monocytes by the addition of 25 ng ml-1 M-CSF and 30 ng ml-1 RANKL as described in Materials and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, KO
Clone
462802
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB4614U-100UG
Alexa Fluor 405
FAB4614V-100UG
Alexa Fluor 488
FAB4614G-100UG
Alexa Fluor 532
FAB4614X
Alexa Fluor 594
FAB4614T-100UG
Alexa Fluor 647
FAB4614R-100UG
Alexa Fluor 700
FAB4614N-100UG
Alexa Fluor 750
FAB4614S-100UG
Allophycocyanin/Cy7
FAB4614APCCY7
Biotin
FAB4614B
CoraFluor 1
FAB4614CL1
DyLight 350
FAB4614D
DyLight 405
FAB4614E
DyLight 488
FAB4614K
DyLight 550
FAB4614L
DyLight 594
FAB4614M
DyLight 650
FAB4614W
DyLight 680
FAB4614Y
DyLight 755
FAB4614Z
HRP
FAB4614H
Janelia Fluor 525
FAB4614JF525
Janelia Fluor 549
FAB4614I
Janelia Fluor 585
FAB4614JF585
Janelia Fluor 635
FAB4614JF635
Janelia Fluor 646
FAB4614J
Janelia Fluor 669
FAB4614JF669
PE/Atto594
FAB4614PEATT594
PE/Cy5.5
FAB4614PECY55
PE/Cy7
FAB4614PECY7
PerCP
FAB4614C
mFluor Violet 450 SE
FAB4614MFV450
mFluor Violet 500 SE
FAB4614MFV500
mFluor Violet 610 SE
FAB4614MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4614-SP
Carrier Free
FAB4614V-100UG
Carrier Free
FAB4614U-100UG
Carrier Free
FAB4614T-100UG
Carrier Free
FAB4614S-100UG
Carrier Free
FAB4614R-100UG
Carrier Free
FAB4614N-100UG
Carrier Free
FAB4614G-100UG
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Calcitonin R Antibody (462802) [Unconjugated] Summary

Immunogen
HEK293 human embryonic kidney cell line transfected with human Calcitonin R
Ala25-Ala474
Accession # NP_001733
Specificity
Detects human Calcitonin R. Stains human Calcitonin R transfectants but not irrelevant transfectants.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CALCR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated 0.25 ug/10^6 cells
Publications
Read Publications using
MAB4614 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calcitonin R Antibody (462802) [Unconjugated]

  • Calcitonin R
  • calcitonin receptor
  • CalcitoninR
  • CALCR
  • CRT
  • CTR
  • CT-R
  • CTR1

Background

Calcitonin receptor (CALCR) is a glycosylated 70 kDa seven-transmembrane G protein-coupled receptor that mediates the hypocalcemic effects of the peptide hormone, calcitonin. CALCR activation inhibits osteoclast-mediated bone resorption and enhances renal calcium excretion. CALCR polymorphisms and mutations have been associated with several bone disorders. Alternative splicing results in the deletion of 16 aa in the first cytoplasmic loop or 23 aa in the first extracellular region. Human CALCR shares 70%‑72% aa sequence identity with mouse and rat CACLR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calcitonin R Antibody (MAB4614)(6)

We have publications tested in 2 confirmed species: Human, Primate - Chlorocebus aethiops (African Green Monkey).

We have publications tested in 3 applications: Flow Cytometry, ICC, IHC.


Filter By Application
Flow Cytometry
(2)
ICC
(3)
IHC
(1)
All Applications
Filter By Species
Human
(5)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB4614 Applications Species
D Steller, A Scheibert, T Sturmheit, SG Hakim Establishment and validation of an in vitro co-culture model for oral cell lines using human PBMC-derived osteoclasts, osteoblasts, fibroblasts and keratinocytes Sci Rep, 2020-10-08;10(1):16861. 2020-10-08 [PMID: 33033302] (Flow Cytometry, Human) Flow Cytometry Human
J Costa-Rodr, MH Fernandes, O Pinho, PRR Monteiro Modulation of human osteoclastogenesis and osteoblastogenesis by lycopene J. Nutr. Biochem., 2018-03-15;57(0):26-34. 2018-03-15 [PMID: 29655028] (ICC, Human) ICC Human
Dal Maso E, Zhu Y, Pham V, Reynolds C, Deganutti G, Hick C, Yang D, Christopoulos A, Hay D, Wang M, Sexton P, Furness S, Wootten D Extracellular loops 2 and 3 of the calcitonin receptor selectively modify agonist binding and efficacy. Biochem Pharmacol, 2018-02-16;150(0):214-244. 2018-02-16 [PMID: 29454620] (Flow Cytometry, Primate - Chlorocebus aethiops (African Green Monkey)) Flow Cytometry Primate - Chlorocebus aethiops (African Green Monkey)
Kasonga A, Deepak V, Kruger M, Coetzee M Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro. PLoS ONE, 2015-04-13;10(4):e0125145. 2015-04-13 [PMID: 25867515] (IHC, Human) IHC Human
Costa-Rodrigues J, Martins EG, Fernandes MH Induced osteoclastogenesis by fluoroquinolones in unstimulated and stimulated human osteoclast precursor cells. Bone, 2012-03-23;51(1):17-27. 2012-03-23 [PMID: 22481084] (ICC, Human) ICC Human
Costa-Rodrigues J, Teixeira CA, Sampaio P, Fernandes MH Characterisation of the osteoclastogenic potential of human osteoblastic and fibroblastic conditioned media. J. Cell. Biochem., 2010-01-01;109(1):205-16. 2010-01-01 [PMID: 19911374] (ICC, Human) ICC Human

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Bioinformatics

Gene Symbol CALCR
Uniprot