CHT1 Antibody (62-2E8)

CHT1 (high affinity choline transporter 1) is a member of sodium:solute symporter (SSF) (TC 2.A.21) family that facilitates sodium/chloride-ion dependent import of choline from extracellular space to the neurons with high affinity. CHT1 is expressed in putamen, spinal cord and medulla, and is specific for cholinergic neurons. Acetyl choline is synthesized from choline and acetyl CoA by choline acetyltransferase in the cytoplasm of cholinergic neurons, and then incorporated into synaptic vesicles by VAChT (vesicular acetylcholine transporter). In the pre-synaptic terminals of cholinergic neurons, CHT1 mediates choline uptake which is the rate limiting step for acetyl-choline biosynthesis and is essential for post-natal viability. CHT1 been shown to be internalized in a clathrin dependent manner, whereas, the synaptic vesicles exocytosis enhances its cell surface expression; a mechanism that facilitates CHT1 functions at cellular surfaces with its predominant localization in the synaptic vesicles in cholinergic neurons. Moreover, Nedd4-2-mediated ubiquitination regulates CHT1 cell surface expression reflecting a possibility that treatments or drugs which inhibit CHT1-Nedd4-2 interactions might be useful for diseases involving decrease in acetylcholine level such as Alzheimer's disease. Moreover, polymorphic variation in CHT1 has been shown to predict early, subclinical measures of carotid atherosclerosis which may aid in understanding cholinergic-vagal processes potentially underlying atherosclerotic risk.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Primate.

We have publications tested in 3 applications: IF/IHC, IHC-Fr, WB.

Showing Publications 1 - 3 of 3.

Publications using NB110-74570	Applications	Species
Misawa H, Fujigaya H, Nishimura T, Moriwaki Y, Okuda T, Kawashima K, Nakata K, Ruggiero AM, Blakely RD, Nakatsu F, Ohno H. Aberrant trafficking of the high-affinity choline transporter in AP-3-deficient mice. Eur J Neurosci;27(12):3109-17. 2008-06-01 [PMID: 18554297] (WB, IF/IHC, Mouse)	WB, IF/IHC	Mouse
Ferguson SM, Savchenko V, Apparsundaram S, Zwick M, Wright J, Heilman CJ, Yi H, Levey AI, Blakely RD. Vesicular localization and activity-dependent trafficking of presynaptic choline transporters. J Neurosci;23(30):9697-709. 2003-10-29 [PMID: 14585997] (IF/IHC, Mouse)	IF/IHC	Mouse
Kus L, Borys E, Ping Chu Y et al. Distribution of high affinity choline transporter immunoreactivity in the primate central nervous system. J Comp Neurol. 2003-08-25 [PMID: 12820166] (IHC-Fr, Primate, Human)	IHC-Fr	Primate, Human

Array NB110-74570

• CHT1 Antibodies
• CHT1 ELISA Kits
• CHT1 Lysate