Novus Biologicals products are now on bio-techne.com

CHMP2B Antibody

Images

 
Western Blot: CHMP2B Antibody [NBP2-15887] - Jurkat whole cell extracts and Jurkat exosome extract (15.4 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP2B antibody diluted at 1:250. ...read more
Immunocytochemistry/ Immunofluorescence: CHMP2B Antibody [NBP2-15887] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: CHMP2B Antibody [NBP2-15887] - Paraffin-embedded human breast carcinoma. CHMP2B antibody diluted at 1:500.
Western Blot: CHMP2B Antibody [NBP2-15887] - Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Mu, Po, Bv, Ch, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

CHMP2B Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CHMP2B. The exact sequence is proprietary.
Localization
Cytoplasm; cytosol; Late endosome membrane
Predicted Species
Mouse (100%), Porcine (99%), Bovine (99%), Rhesus Macaque (99%), Xenopus (94%), Chicken (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHMP2B
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (94%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for CHMP2B Antibody

  • charged multivesicular body protein 2b
  • CHMP2.5
  • CHMP2.5VPS2BVPS2-2
  • CHMP2B
  • chromatin modifying protein 2B
  • Chromatin-modifying protein 2b
  • DKFZP564O123
  • DMT1
  • hVps2-2
  • Vacuolar protein sorting-associated protein 2-2
  • vacuolar protein-sorting-associated protein 2-2
  • VPS2 homolog B
  • Vps2-2
  • VPS2B

Background

This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00004891-M01
Species: Bv, Hu, Mu, Po
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP1-21502
Species: Bv, Hu, Mu, Po, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, WB
2914-HT
Species: Hu
Applications: BA
NBP2-25162
Species: Bv, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, PLA, WB
NB300-914
Species: Hu, Mu
Applications: IHC, IHC-P, PEP-ELISA
DHP250
Species: Hu
Applications: ELISA
NBP1-85483
Species: Hu
Applications: IHC, IHC-P
H00023435-M01
Species: Ba, Pp, Hu, I, Pm, Mu, Rt
Applications: ELISA, Func, ICC/IF, IHC, IHC-P, IP, KD, WB
MAB8400
Species: Hu
Applications: CyTOF-ready, Flow, WB
H00003077-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
NB100-565
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KO, WB
NBP2-80524
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB100-1558
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-Fr, IP, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CHMP2B Antibody (NBP2-15887) (0)

There are no publications for CHMP2B Antibody (NBP2-15887).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CHMP2B Antibody (NBP2-15887) (0)

There are no reviews for CHMP2B Antibody (NBP2-15887). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CHMP2B Antibody (NBP2-15887) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CHMP2B Products

Research Areas for CHMP2B Antibody (NBP2-15887)

Find related products by research area.

Blogs on CHMP2B

There are no specific blogs for CHMP2B, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CHMP2B Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CHMP2B
Uniprot