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CHMP2B Antibodies

We offer CHMP2B Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each CHMP2B Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CHMP2B Antibodies can be used in a variety of model species: Human. Use the list below to choose the CHMP2B Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CHMP2B polyclonal antibodies and browse our CHMP2B monoclonal antibody catalog.

Alternate Names for CHMP2B Antibodies

anti-CHMP2B antibody, anti-charged multivesicular body protein 2b antibody, anti-CHMP2.5VPS2BVPS2-2 antibody, anti-chromatin modifying protein 2B antibody, anti-Chromatin-modifying protein 2b antibody, anti-DKFZP564O123 antibody, anti-DMT1 antibody, anti-hVps2-2 antibody, anti-Vacuolar protein sorting-associated protein 2-2 antibody, anti-vacuolar protein-sorting-associated protein 2-2 antibody, anti-VPS2 homolog B antibody, anti-Vps2-2 antibody

12 Results for "chmp2b" in Primary Antibodies

MAB7509

CHMP2B Antibody (791521) [Unconjugated]

(3 Publications)

100 μg

MAB7509

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CHMP2B Monoclonal Antibody (Catalog # MAB7509) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CHMP2B at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CHMP2B was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human CHMP2B Monoclonal Antibody (Catalog # MAB7509) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Staining is shown at both high (upper panel) and low (lower panel) magnification, and specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of U2OS human osteosarcoma cell line and CHMP2B knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 1.25 µg/mL of Mouse Anti-Human CHMP2B Monoclonal Antibody (Catalog # MAB7509) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for CHMP2B at approximately 28 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7509-SP

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AF7509

CHMP2B Antibody [Unconjugated]

(1 Publication)

100 μg

AF7509

Western blot shows lysates of human brain (hippocampus) tissue, human placenta tissue, rat brain tissue, and mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat CHMP2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7509) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHMP2B at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CHMP2B was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Sheep Anti-Human/Mouse/Rat CHMP2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7509) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7509-SP

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NBP2-15887

CHMP2B Antibody

0.1 ml

NBP2-15887

Western Blot: CHMP2B Antibody [NBP2-15887] - Jurkat whole cell extracts and Jurkat exosome extract (15.4 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP2B antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: CHMP2B Antibody [NBP2-15887] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: CHMP2B Antibody [NBP2-15887] - Paraffin-embedded human breast carcinoma. CHMP2B antibody diluted at 1:500.

Western Blot: CHMP2B Antibody [NBP2-15887] - Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE gel, diluted at 1:1000.

Western Blot: CHMP2B Antibody [NBP2-15887] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP2B antibody (NBP2-15887) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: CHMP2B Antibody [NBP2-15887] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE NBP2-15887 diluted at 1:1000

Reactivity:

Hu, Mu, Mu, Po, Bv, Ch, RM, Xp

Species Glossary

Reactivity: Hu, Mu, Mu, Po, Bv, Ch, RM, Xp

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-15886

CHMP2B Antibody

0.1 ml

NBP2-15886

Western Blot: CHMP2B Antibody [NBP2-15886] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: CHMP2B Antibody [NBP2-15886] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using CHMP2B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Western Blot: CHMP2B Antibody [NBP2-15886] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP2B antibody (NBP2-15886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Po, Bv, Ch, RM, Xp

Species Glossary

Reactivity: Hu, Mu, Po, Bv, Ch, RM, Xp

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-15707

CHMP2B Antibody (3L9O5)

100 ul

NBP3-15707

Western Blot: CHMP2B Antibody (3L9O5) [NBP3-15707] - Western blot analysis of extracts of various cell lines, using CHMP2B antibody (NBP3-15707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: CHMP2B Antibody (3L9O5) [NBP3-15707] - Immunohistochemistry of paraffin-embedded mouse testis using CHMP2B Rabbit mAb (NBP3-15707) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-22344

CHMP2B Antibody (SR1463)

100 ul

NBP3-22344

Western Blot: CHMP2B Antibody (SR1463) [NBP3-22344] - Western blot analysis using NBP3-22344 in A431 cell lysate.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-29086

CHMP2B Antibody

100 μg

NBP3-29086

Immunohistochemistry;CHMP2B Antibody[NBP3-29069]Detection of human CHMP2B by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHMP2B used at a dilution of 1:1,000 . Detection: DAB. Counterstain: IHC Hematoxylin .

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-84482

CHMP2B Antibody

(1 Publication)

0.1 ml

NBP1-84482

Immunohistochemistry-Paraffin: CHMP2B Antibody [NBP1-84482] - Staining of human lymph node shows strong cytoplasmic positivity in subsets of lymphoid cells outside reaction centra.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-56956

CHMP2B Antibody

100 ul

NBP2-56956

Immunocytochemistry/Immunofluorescence: CHMP2B Antibody [NBP2-56956] - Staining of human cell line U-2 OS shows localization to cytosol.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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H00025978-M01

CHMP2B Antibody (2H6-1E6)

0.1 mg

H00025978-M01

Sandwich ELISA: CHMP2B Antibody (2H6-1E6) [H00025978-M01] - Detection limit for recombinant GST tagged CHMP2B is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00025978-M02

CHMP2B Antibody (M1)

0.1 mg

H00025978-M02

Sandwich ELISA: CHMP2B Antibody (M1) [H00025978-M02] - Detection limit for recombinant GST tagged CHMP2B is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-29240

CHMP2B Antibody

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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