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CEBP Beta Antibody

Images

 
Western Blot: CEBP Beta Antibody [NBP2-26206] - Staining of Human Skin (A) and Pig Lung (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: CEBP Beta Antibody [NBP2-26206] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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CEBP Beta Antibody Summary

Immunogen
Peptide with sequence C-DHERAIDFSPYLEP corresponding to internal region according to NP_005185.2.
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CEBPB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in NIH3T3 lysates (calculated MW of 31.5 kDa band according to mouse NP_034013.1).
Publications
Read Publication using NBP2-26206.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CEBP Beta Antibody

  • C/EBP beta
  • C/EBP-beta
  • CCAAT/enhancer binding protein (C/EBP), beta
  • CCAAT/enhancer-binding protein beta
  • CRP2
  • IL6DBP
  • interleukin 6-dependent DNA-binding protein
  • LAPTCF-5
  • Liver activator protein
  • liver-enriched transcriptional activator protein
  • MGC32080
  • NF-IL6
  • Nuclear factor NF-IL6
  • nuclear factor of interleukin 6
  • TCF5NFIL6
  • Transcription factor 5

Background

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth and differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). A number of serine targets of PKA have been identified in C/EBP, suggesting its activities are post-translationally regulated by cAMP (2). Even though, it has been show that C/EBP beta occurs predominantly as a heterodimer (3). C/EBP beta gamma readily heterodimerize with each other as well as with C/EBP alpha (4). Additionally, 3 isoforms of C/EBP beta can be detected (LAP, LAP,LIP)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEBP Beta Antibody (NBP2-26206)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional CEBP Beta Products

Blogs on CEBP Beta.

Transcription Factor Antibodies Used In Landmark Evolutionary Study
We at Novus Biologicals offer a full antibody database targeted to transcription factor research. Recently, CEBP antibodieswere used in a research study exploring the evolution of gene regulation in various vertebrates. The results revealed surprising...  Read full blog post.

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Bioinformatics

Gene Symbol CEBPB
Entrez