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Human CEBP alpha ELISA Kit (Colorimetric)

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ELISA: Human CEBP alpha ELISA Kit (Colorimetric) [NBP2-70024] - Samples were spiked with high concentrations of Human CEBP alpha and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Human CEBP alpha ELISA Kit (Colorimetric) [NBP2-70024] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Human CEBP alpha ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human C/EBP(alpha) in samples. No significant cross-reactivity or interference between Human C/EBP(alpha) and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.39%
Intra-Assay
CV% < 4.2%
Spike Recovery
84-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CEBPA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human CEBP alpha ELISA Kit (Colorimetric)

  • C/EBP alpha
  • C/EBP-alpha
  • CCAAT/enhancer binding protein (C/EBP), alpha
  • CCAAT/enhancer-binding protein alpha
  • CEBP alpha
  • CEBP
  • CEBPA

Background

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). C/EBP alpha (2) can form heterodimers with related protein C/EBP beta and gamma. C/EBP alpha directly interacts with CDK2and CDK4 and arrests cell proliferation by inhibiting these kinases (3). C/EBP alpha has been shown to bind to the promoter and modulate the expression of leptin, a protein that plays an important role in body weight homeostasis (4). C/EBP alpha also has prominent antimitotic activity and may play a role of a tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Transcription Factor Antibodies Used In Landmark Evolutionary Study
We at Novus Biologicals offer a full antibody database targeted to transcription factor research. Recently, CEBP antibodieswere used in a research study exploring the evolution of gene regulation in various vertebrates. The results revealed surprising...  Read full blog post.

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Bioinformatics

Gene Symbol CEBPA