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Human Cdc23 ELISA Kit (Colorimetric)

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ELISA: Human Cdc23 ELISA Kit (Colorimetric) [NBP2-75167] - Samples were spiked with high concentrations of Human Cdc23 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human Cdc23 ELISA Kit (Colorimetric) [NBP2-75167] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Human Cdc23 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CDC23 in samples. No significant cross-reactivity or interference between Human CDC23 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.5%
Intra-Assay
CV% < 4.83%
Spike Recovery
83-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CDC23

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Cdc23 ELISA Kit (Colorimetric)

  • ANAPC8anaphase promoting complex subunit 8
  • Anaphase-promoting complex subunit 8
  • APC8CDC23 (cell division cycle 23, yeast, homolog)
  • cell division cycle 23 homolog (S. cerevisiae)
  • cell division cycle protein 23 homolog
  • CUT23
  • Cyclosome subunit 8

Background

CDC23/APC8 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CDC23