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CD90/Thy1 Antibody

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Western Blot: CD90/Thy1 Antibody [NBP1-51963] - (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: CD90/Thy1 Antibody [NBP1-51963] - (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CD90/Thy1 Antibody [NBP1-51963] - (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Po, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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CD90/Thy1 Antibody Summary

Immunogen
Peptide with sequence C-PEHTYRSRTNFTSKY, from the internal region of the protein sequence according to NP_006279.2.
Marker
Mesenchymal Cell Marker
Predicted Species
Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
THY1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA detection limit 1:64000
  • Western Blot 0.1-0.3 ug/ml
Application Notes
This antibody works in ELISA and Western Blot. Approx 26kDa band observed in Human Brain (Cerebellum) and Human Tonsil lysates (calculated MW of 17.9kDa according to NP_006279.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex).
Publications
Read Publications using NBP1-51963.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD90/Thy1 Antibody

  • CD90 antigen
  • CD90
  • CDw90
  • FLJ33325
  • Thy-1 antigen
  • Thy-1 cell surface antigen
  • thy-1 membrane glycoprotein
  • Thy-1 T-cell antigen
  • Thy1

Background

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD90/Thy1 Antibody
NBP1-51963
CD90/Thy1 Antibody
Species: Hu, Po, Ca
Applications: WB, ELISA, IHC

Publications for CD90/Thy1 Antibody (NBP1-51963)(2)


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FAQs for CD90/Thy1 Antibody (NBP1-51963). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.

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Bioinformatics

Gene Symbol THY1
Entrez
Uniprot