CD8 Antibodies
CD8 is a cell-surface glycoprotein that present on T-cells as a co-receptor for MHC Class I, playing a roll in T-cell activation and immune response. Human CD8α is processed as 235 amino acids with a theoretical molecular weight of ~26 kDa. We offer antibodies to detect CD8 that have been cited in 40+ publications. Browse our catalog of monoclonal, polyclonal, and CD8-conjugated antibodies. Validated research applications for our CD8 antibodies include Blocking/Neutralizing, CyTOF-ready, Dual RNA-scope ISH-IHC, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC-Frozen, IHC-Paraffin, IP, Sandwich ELISA Capture, and Western Blot. Our CD8 antibodies can be used in a variety of model species including Human, Mouse, Rat, Bovine, Canine, Chicken, Feline, Goat, Primate, Rabbit, and Sheep, to name a few. Each CD8 antibody is fully covered by our Guarantee+. Have confidence in choosing the CD8 antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products.
Alternate Names for CD8 Antibodies
anti-CD8 antibody, anti-CD8A antibody
63 Results for "cd8" in Primary Antibodies
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Flow-Cytometry-NBP1-49045-img0014.jpg "Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.")
![Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-Paraffin-NBP1-49045-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0006.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Staining of peripheral blood leukocytes.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Flow-Cytometry-NBP1-49045-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0011.jpg)
![Immunohistochemistry: CD8 Antibody (53-6.7) [NBP1-49045] - Histopathological analysis of spinal cord in EAE mice. Representative spinal cord sections from wild-type (WT), Asic1-/- , and Asic2-/- mice at 45 days after immunization. Sections of the spinal cord anterior horn were stained with hematoxylin and eosin (E&H) to assess inflammation and immunostained for MCHII, CD4+, CD8+ infiltrating cells. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/ejn.14302), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-NBP1-49045-img0016.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) - BSA Free [NBP1-49045] - Analysis of isolated mouse splenic CD8+ T cells using CD8 antibody (53-6.7) [Allophycocyanin] (NBP1-49045APC). Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-BSA-Free-Flow-Cytometry-NBP1-49045-img0017.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-Frozen-NBP1-49045-img0008.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-Frozen-NBP1-49045-img0015.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - Analysis of fixed murine splenocytes by multiple staining.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Flow-Cytometry-NBP1-49045-img0009.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, and stained for or Ccdc88b (red) and CD8 (green). Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030607/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD8-Antibody-Immunohistochemistry-Frozen-NBP2-29475-img0002.jpg "Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, and stained for or Ccdc88b (red) and CD8 (green). Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030607/) licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. aCcdc88b mRNA expression in distal colons of NT (n = 3) or DSS-treated WT mice (n = 3 for each time point) at the indicated days. Data represent expression relative to hprt±SEM (n = 3). *P < 0.05, **P < 0.01 (two-tailed Student’s t-test) & are representative of one experiment. b Representative immunoblots for Ccdc88b protein detected in extracts from distal colons from NT or DSS-treated WT & Ccdc88Bmut mutant mice at indicated time points, & densitometric quantification of Ccdc88b immunoblot normalized to beta -actin±SEM (n = 3) (representative of one of two independent experiments); **P < 0.01 (two-tailed Student’s t-test). c Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at indicated times, & stained with antibodies against Ccdc88b (red), CD45 (green), & E-Cadherin (purple) & nuclei staining DAPI (blue). Scale bars, 100 μm. d Representative FACS plots & quantification of lamina propria cells stained for CD45 & for Ccdc88b antibodies for NT (n = 3) & DSS-treated WT mice (n = 4 for each time point) at indicated time points, data are representative of one experiment. e Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, & stained for Ccdc88b (red), CD3 (green) & CD11b (green); nuclei are stained with DAPI (blue). Scale bars, 100 μm. f same as in e for Ccdc88b (red), CD4 (green) & CD8 (green). Scale bars, 100 μm Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-01381-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-29475_rabbit-polyclonal-cd8-antibody-imgenex-abx-160a-20ug-310202416113534.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using PerCP conjugate of NBP2-25195. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0015.jpg "Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using PerCP conjugate of NBP2-25195. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.")
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25195. Staining of CD8 alpha in human PBMC using CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0012.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - Analysis using the DyLight 405 conjugate of NBP2-25195. Staining of CD8 alpha in human PBMCs using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0013.jpg)
![Flow (Cell Surface): CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using the PE conjugate of NBP2-25195. Staining of Human peripheral blood mononuclear cells. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cell-Surface-NBP2-25195-img0014.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-25195] - Analysis using the PE conjugate of NBP2-25195. Staining of CD8 in 10^6 human PBMC using 10 ul (0.1 ug) of this antibody. The shaded histogram represents human PBMC alone; green represents mouse isotype control, red represents human CD8 this antibody.](http://images.novusbio.com/fullsize/nbp2-25195_mouse-monoclonal-cd8-antibody-rpa-t8-imgenex-img-5917a-25ug-1772024104463.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-25195] - Analysis using the FITC conjugate of NBP2-25195. Staining of CD8 in human PBMCs using 0.25 ug of (clone RPA-T8) per approximately 1x10^6 cells. Green represents isotype control this antibody, red represents anti-CD8 FITC antibody.](http://images.novusbio.com/fullsize/nbp2-25195_mouse-monoclonal-cd8-antibody-rpa-t8-imgenex-img-5917a-25ug-17720241044620.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of a mouse lymph node cryosection with rat anti-mouse CD19, clone 6D5 green in A and rat anti-mouse CD8, red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power](http://images.novusbio.com/fullsize/CD8-Antibody-YTS105-18-Immunohistochemistry-Frozen-NB200-578-img0006.jpg "Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of a mouse lymph node cryosection with rat anti-mouse CD19, clone 6D5 green in A and rat anti-mouse CD8, red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power")
![Flow Cytometry: CD8 Antibody (YTS105.18) [NB200-578] - Staining of mouse spleen cells.](http://images.novusbio.com/fullsize/CD8-Antibody-YTS105-18-Flow-Cytometry-NB200-578-img0003.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of Mouse lymph node cryosection with CD8 Antibody and HRP-conjugated goat anti-rat IgG. Low power magnification.](http://images.novusbio.com/fullsize/CD8-Antibody-YTS105-18-Immunohistochemistry-Frozen-NB200-578-img0005.jpg)
![Immunohistochemistry: CD8 Antibody (12.C7) [NB100-64021] - scAAV8G9-optHLA-G Combo Prevents Cornea Burn-induced Vascularization and Cytotoxic T-cell Infiltration. Rabbit cornea sections were acquired 60 days following the injection of indicated vectors into burn corneas and stained for an endothelial cell marker (CD31), T cell markers, transgene abundance, and a-SMA in the indicated treatment groups. Scale bars=5um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-18002-9), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD8-Antibody-12-C7-Immunohistochemistry-NB100-64021-img0002.jpg "Immunohistochemistry: CD8 Antibody (12.C7) [NB100-64021] - scAAV8G9-optHLA-G Combo Prevents Cornea Burn-induced Vascularization and Cytotoxic T-cell Infiltration. Rabbit cornea sections were acquired 60 days following the injection of indicated vectors into burn corneas and stained for an endothelial cell marker (CD31), T cell markers, transgene abundance, and a-SMA in the indicated treatment groups. Scale bars=5um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-18002-9), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CD8 Antibody (12.C7) [NB100-64021] - Human bile duct cancer tissue. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-12-C7-Immunohistochemistry-Paraffin-NB100-64021-img0003.jpg)
![Flow Cytometry: CD8 alpha Antibody (12.C7) [NB100-64021] - Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD8 Alpha followed by Goat anti Mouse IgG (H/L):FITC](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-12-C7-Flow-Cytometry-NB100-64021-img0001.jpg)
![Flow Cytometry: CD8 Antibody (OX-8) [NBP2-12523] - Separation of rat CD8a positive thymocytes (red-filled) from CD8a negative thymocytes (black-dashed) in flow cytometry analysis (surface staining) of rat thymocyte suspension stained using anti-rat CD8 (OX-8) purified antibody (concentration in sample 0.32 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD8-Antibody-OX-8-Flow-Cytometry-NBP2-12523-img0002.jpg "Flow Cytometry: CD8 Antibody (OX-8) [NBP2-12523] - Separation of rat CD8a positive thymocytes (red-filled) from CD8a negative thymocytes (black-dashed) in flow cytometry analysis (surface staining) of rat thymocyte suspension stained using anti-rat CD8 (OX-8) purified antibody (concentration in sample 0.32 ug/ml) GAM APC.")
![Flow Cytometry: CD8 Antibody (OX-8) [NBP2-12523] - Flow cytometry surface staining pattern of rat thymocyte suspension stained using anti-rat CD8 (OX-8) purified antibody (concentration in sample 0.32 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD8-Antibody-OX-8-Flow-Cytometry-NBP2-12523-img0001.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody [NBP2-34039] - CD8 alpha Antibody [NBP2-34039] - Analysis in human spleen and skeletal muscle tissues. Corresponding CD8A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-34039-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD8 Antibody [NBP2-34039] - CD8 alpha Antibody [NBP2-34039] - Analysis in human spleen and skeletal muscle tissues. Corresponding CD8A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD8 alpha Antibody [NBP2-34039] - Staining of human spleen shows moderate to strong positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-34039-img0003.jpg)
![Immunohistochemistry-Paraffin: CD8 alpha Antibody [NBP2-34039] - Staining of human lymph node shows moderate to strong positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-34039-img0004.jpg)
![Immunohistochemistry-Paraffin: CD8 alpha Antibody [NBP2-34039] - Staining of human small intestine shows moderate to strong positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-34039-img0005.jpg)
![Immunohistochemistry-Paraffin: CD8 alpha Antibody [NBP2-34039] - Staining of human skeletal muscle shows no cytoplasmic positivity as expected.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-34039-img0006.jpg)
![Dual RNAscope ISH-IHC: CD8 Antibody (4B11) [NB100-65729] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD8 mRNA (ACD RNAScope probe, catalog # 560391; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using mouse monoclonal (Novus catalog # NB100-65729) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC001) and green HRP chromogen (ACD Catalog # 322500).](http://images.novusbio.com/fullsize/CD8-Antibody-4B11-Dual-RNAscope-ISH-IHC-NB100-65729-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD8 Antibody (4B11) [NB100-65729] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD8 mRNA (ACD RNAScope probe, catalog # 560391; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using mouse monoclonal (Novus catalog # NB100-65729) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC001) and green HRP chromogen (ACD Catalog # 322500).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD8 Antibody (4B11) [NB100-65729] - Analysis of formalin fixed, paraffin-embedded human tonsil with CD8 Antibody (4B11). Magnification: 40X.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-4B11-Immunohistochemistry-Paraffin-NB100-65729-img0002.jpg)