![Flow Cytometry: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Using the Anti-CD45RA antibody OTH-74D4 (Ab01502). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with CD45RA antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of OTH-74D4 (Ab01502-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD45RA-Antibody-OTH-74D4-Chimeric-Flow-Cytometry-NBP3-12063-img0001.jpg "Flow Cytometry: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Using the Anti-CD45RA antibody OTH-74D4 (Ab01502). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with CD45RA antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of OTH-74D4 (Ab01502-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF |
| Clone | OTH-74D4 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was raised by immunising mice with an EBV-transformed B cell line from a patient with MHC class II deficiency. |
| Specificity | OTH-74D4 antibody recognises CD45RA, which is an CD45 isoform containing only protein A region.The CD45RA antigen is expressed mostly on naive CD4+, CD8+ and gd+ T and on most B and NK lymphocytes. Generally the CD45RA antigen density decreases upon activation. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PTPRC |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CD45RA Antibody (NBP3-12063)Find related products by research area.
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| Gene Symbol | PTPRC |
![Immunofluorescence: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Staining of Jukat cells with anti-CD45RA (Ab01502) OTH-74D4 Immunofluorescence analysis of paraformaldehyde fixed Jukat cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of OTH-74D4 (Ab01502-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01502-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/CD45RA-Antibody-OTH-74D4-Chimeric-Immunofluorescence-NBP3-12063-img0002.jpg "Immunofluorescence: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Staining of Jukat cells with anti-CD45RA (Ab01502) OTH-74D4 Immunofluorescence analysis of paraformaldehyde fixed Jukat cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of OTH-74D4 (Ab01502-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01502-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.")
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