![Immunocytochemistry/Immunofluorescence: CD44 Antibody (MEM-263) [NB500-481] - analysis of CD44 in 4% paraformaldehyde fixed Caov-3 cells (adherent human ovarian cancer cells) using anti-Cd44 antibody. The primary antibody was used at a dilution of 1:1000 in 2% goat serum in PBS and incubated for 2 hours at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Immunocytochemistry-Immunofluorescence-NB500-481-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD44 Antibody (MEM-263) [NB500-481] - analysis of CD44 in 4% paraformaldehyde fixed Caov-3 cells (adherent human ovarian cancer cells) using anti-Cd44 antibody. The primary antibody was used at a dilution of 1:1000 in 2% goat serum in PBS and incubated for 2 hours at room temperature. Image from verified customer review.")
| Reactivity | Hu, Po, CaSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | MEM-263 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | COS-7 cells, African Green Monkey (NM_000610.3). |
| Marker | Cell Membrane Marker |
| Specificity | The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. HLDA III; WS Code T 155 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD44 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25481699). This antibody is CyTOF ready. |
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| Theoretical MW | 95 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Joelle Sacks |
IF | Human | 12/16/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![CD44 Antibody (MEM-263) [NB500-481] - Analysis of CD44 in HeLa cells using anti-CD44 (MEM-263) purified; non-reducing conditions.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-NB500-481-img0009.jpg "CD44 Antibody (MEM-263) [NB500-481] - Analysis of CD44 in HeLa cells using anti-CD44 (MEM-263) purified; non-reducing conditions.")
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - A surface stain was performed on hPBMCs with CD44 (MEM-263) antibody NB500-481AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0003.jpg "Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - A surface stain was performed on hPBMCs with CD44 (MEM-263) antibody NB500-481AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) APC conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0007.jpg "Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) APC conjugate.")
![Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Expression of CD44 and CK18 in multiple clones of ovarian cancer cells. CD44+ cells also express CK18 as determined by western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jo/2011/620523/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Western-Blot-NB500-481-img0008.jpg "Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Expression of CD44 and CK18 in multiple clones of ovarian cancer cells. CD44+ cells also express CK18 as determined by western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jo/2011/620523/) licensed under a CC-BY license.")
![Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Western-Blot-NB500-481-img0002.jpg "Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.")
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Analysis of human peripheral blood (lymphocyte gate) using anti-CD44 (MEM-263) PE conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0004.jpg "Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Analysis of human peripheral blood (lymphocyte gate) using anti-CD44 (MEM-263) PE conjugate.")
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) FITC conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0005.jpg "Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) FITC conjugate.")
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![Flow Cytometry: CD44 Antibody (MEM-263) [Alexa Fluor 647] [NB500-481AF647] - A surface stain was performed on hPBMCs with CD44 (MEM-263) antibody NB500-481AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](https://images.novusbio.com/images/CD44-Antibody-MEM-263-Alexa-Fluor-647-Flow-Cytometry-NB500-481AF647-img0002.jpg "Flow Cytometry: CD44 Antibody (MEM-263) [Alexa Fluor 647] [NB500-481AF647] - A surface stain was performed on hPBMCs with CD44 (MEM-263) antibody NB500-481AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
