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CD44 Antibody (Hermes-1) - Azide and BSA Free

Images

 
Western Blot: CD44 Antibody (Hermes-1) [NBP2-22530] - of Hela cell lysates.
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (Hermes-1) [NBP2-22530] - Analysis of CD44 (green) showing staining in the membrane of Hela cells (right) compared to a negative control without primary antibody ...read more
Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 (Hermes-1) Antibody NBP2-22530R (blue) and a matched isotype control (orange). Cells were incubated in an ...read more
Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - Analysis of CD44 in PBMC cells (green) compared to an isotype control (blue).
Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 [Hermes-1] Antibody NBP2-22530AF488 (blue) and a matched isotype control (orange). Cells were incubated in ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Gt, Rb, ShSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, B/N, CyTOF-ready
Clone
Hermes-1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

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CD44 Antibody (Hermes-1) - Azide and BSA Free Summary

Immunogen
80 - 95 kDa lymphocyte surface glycoprotein H-CAM (CD44).
Marker
Cell Membrane Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CD44
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:200
  • Immunohistochemistry 1:10 - 1:2000
  • Immunoprecipitation 1:10 - 1:50
  • Western Blot 1:1 - 1:100
Publications
Read Publications using
NBP2-22530 in the following applications:

  • B/N
    2 publications
  • 1 publication
  • 3 publications
  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD44 Antibody (Hermes-1) - Azide and BSA Free

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • MUTCH-I
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Background

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD44 Antibody (NBP2-22530)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 5 applications: B/N, ChIP, FLOW, IHC-P, WB.


Filter By Application
B/N
(2)
ChIP
(1)
FLOW
(3)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rabbit
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP2-22530 Applications Species
Sawaki D, Zhang Y, Mohamadi A et al. Osteopontin promotes age-related adipose tissue remodeling through senescence-associated macrophage dysfunction JCI insight 2023-04-24 [PMID: 37092554] (ChIP) ChIP
W Niu, TC Lim, A Alshihri, R Rajappa, L Wang, M Kurisawa, M Spector Platelet-Derived Growth Factor Stimulated Migration of Bone Marrow Mesenchymal Stem Cells into an Injectable Gelatin-Hydroxyphenyl Propionic Acid Matrix Biomedicines, 2021-02-17;9(2):. 2021-02-17 [PMID: 33671438]
Reithmeier A, Panizza E, Krumpel M et al. Tartrate-resistant acid phosphatase (TRAP/ACP5) promotes metastasis-related properties via TGF beta 2/T beta R and CD44 in MDA-MB-231 breast cancer cells BMC Cancer 2017-09-15 [PMID: 28915803] (B/N, Human) B/N Human
Lakshminarayan R, Wunder C, Becken U et al. Galectin-3 drives glycosphingolipid-dependent biogenesis of clathrin-independent carriers Nat Cell Biol. 2014-05-18 [PMID: 24837829] (WB, Human) WB Human
Reithmeier A Tartrate-resistant acid phosphatase/ACP5 as a driver of cancer: Dissection of its oncogenic mechanisms and indentification of small molecule inhibitors Thesis 2017-12-15 (B/N, Human) B/N Human
Chatterjee S, Patra D, Ghosh P et al. Activity of ROCKII not ROCKI promotes pulmonary metastasis of melanoma cells via modulating Smad2/3-MMP9 and FAK-Src-VEGF signalling Cellular signalling 2022-06-17 [PMID: 35718242] (IHC-P, FLOW, Mouse) IHC-P, FLOW Mouse
Zhang X, Du L, Zhao N et al. Effects of bone marrow mesenchymal stem cell-derived exosomes on bone formation on titanium Research Square 2020-11-05 [PMID: 33239091] (FLOW, Rabbit)

Details:
Rabbit bone marrow mesenchymal stem cells under went flow cytometry.
FLOW Rabbit
Chen G, Zhang W, Zhang K et al Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits Stem Cells Int 2020-11-02 [PMID: 33133195] (FLOW, Rabbit)

Details:
Citation using the Allophycocyanin version of this antibody.
FLOW Rabbit

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD44 Antibody (NBP2-22530). (Showing 1 - 1 of 1 FAQs).

  1. I need to do a neutralization assay with this Antibody in cell culture. Could you send me the best concentration to use?
    • Although we give recommended dilutions for the other applications in which this antibody is validated, the dilution used for neutralization is assay dependent, and you will need to determine this yourself. Cell type, seeding density and the assay set up will all play a part in deciding this concentration. We recommend that you titrate the antibody for use in your experiment using appropriate negative and positive controls.

Secondary Antibodies

 

Isotype Controls

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Research Areas for CD44 Antibody (NBP2-22530)

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Blogs on CD44.

Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The Osteopontin Antibody and Hepatic Research
Antibody suppliers, such as us at Novus Biologicals, supply a wide range of cell marker products, among them the Osteopontin antibody. Recently, the osteopontin antibody has proven useful in hepatic cancer research.Osteopontin (OPN) is mainly expres...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

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Bioinformatics

Gene Symbol CD44
Entrez
Uniprot