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CD44 Antibody (69-S5)

Images

 
Canine blood‑derived monocytes were stained with Mouse Anti-Canine/Equine CD44 Monoclonal Antibody (Catalog # MAB5449, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more
Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Canine/Equine CD44 Monoclonal Antibody (Catalog # MAB5449, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), ...read more

Product Details

Summary
Reactivity Ca, EqSpecies Glossary
Applications Flow, CyTOF-ready
Clone
69-S5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CD44 Antibody (69-S5) Summary

Immunogen
Canine marrow cells
Accession # Q28284
Specificity
Detects canine and equine CD44 in Flow Cytometry.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD44
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
MAB5449 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD44 Antibody (69-S5)

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • MUTCH-I
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Background

Canine CD44 is a ubiquitously expressed 85‑90 kDa transmembrane glycoprotein that binds to hyaluronan and is involved in matrix adhesion, lymphocyte activation, and lymph node homing. The CD44 protein is expressed as a family of molecular isoforms generated by alternate splicing and variable posttranslational modification. Within the N‑terminal invariant portion of the ECD (aa 14‑191), canine CD44 shares 90%, 83%, and 82% identity with human, mouse, and rat CD44, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD44 Antibody (MAB5449)(10)

We have publications tested in 2 confirmed species: Canine, Equine.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(8)
All Applications
Filter By Species
Canine
(1)
Equine
(6)
All Species
Showing Publications 1 - 10 of 10.
Publications using MAB5449 Applications Species
Katarzyna Kornicka-Garbowska, Lynda Bourebaba, Michael Röcken, Krzysztof Marycz Inhibition of protein tyrosine phosphatase improves mitochondrial bioenergetics and dynamics, reduces oxidative stress, and enhances adipogenic differentiation potential in metabolically impaired progenitor stem cells Cell Communication and Signaling 2021-11-03 [PMID: 34732209]
Bourebaba L, Kornicka-Garbowska K, Al Naem M et al MSI-1436 improves EMS adipose derived progenitor stem cells in the course of adipogenic differentiation through modulation of ER stress, apoptosis, and oxidative stress Stem Cell Res Ther 2/4/2021 [PMID: 33536069]

Details:
Citation using the Allophycocyanin version of this antibody.
L Bourebaba, I Michalak, M Baouche, K Kucharczyk, K Marycz Cladophora glomerata methanolic extract promotes chondrogenic gene expression and cartilage phenotype differentiation in equine adipose-derived mesenchymal stromal stem cells affected by metabolic syndrome Stem Cell Res Ther, 2019-12-17;10(1):392. 2019-12-17 [PMID: 31847882] (Flow Cytometry, Equine) Flow Cytometry Equine
K Marycz, JMI Houston, C Weiss, M Röcken, K Kornicka 5-Azacytidine and Resveratrol Enhance Chondrogenic Differentiation of Metabolic Syndrome-Derived Mesenchymal Stem Cells by Modulating Autophagy Oxid Med Cell Longev, 2019-05-12;2019(0):1523140. 2019-05-12 [PMID: 31214275] (Flow Cytometry, Equine) Flow Cytometry Equine
K Kornicka, J Sz?apka-Ko, A ?mieszek, K Marycz 5-Azacytydine and resveratrol reverse senescence and ageing of adipose stem cells via modulation of mitochondrial dynamics and autophagy J. Cell. Mol. Med., 2018-10-28;23(1):237-259. 2018-10-28 [PMID: 30370650] (Flow Cytometry, Equine) Flow Cytometry Equine
K Marycz, K Kornicka, JM Irwin-Hous, C Weiss Combination of resveratrol and 5-azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells J. Cell. Mol. Med., 2018-07-12;0(0):. 2018-07-12 [PMID: 29999247] (Flow Cytometry, Equine) Flow Cytometry Equine
K Marycz, C Weiss, A ?mieszek, K Kornicka Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses Stem Cells Int, 2018-06-06;2018(0):5340756. 2018-06-06 [PMID: 29977307] (Flow Cytometry, Equine) Flow Cytometry Equine
K Marycz, K Kornicka, J Grzesiak, A ?mieszek, J Sz?apka Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation Oxid Med Cell Longev, 2016-12-08;2016(0):3718468. 2016-12-08 [PMID: 28053691] (Flow Cytometry) Flow Cytometry
Equine metabolic syndrome impairs adipose stem cells osteogenic differentiation by predominance of autophagy over selective mitophagy J Cell Mol Med, 2016-09-14;0(0):. 2016-09-14 [PMID: 27629697] (Flow Cytometry, Equine) Flow Cytometry Equine
Clark K, Kol A, Shahbenderian S, Granick J, Walker N, Borjesson D Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype. Stem Cell Rev, 2016-04-01;12(2):245-56. 2016-04-01 [PMID: 26638159] (Flow Cytometry, Canine) Flow Cytometry Canine

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Isotype Controls

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Bioinformatics

Gene Symbol CD44
Uniprot