CD44 Antibody (2F10) [Phycoerythrin] - v6

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1-3). The standard or hematopoietic form, CD44H, does not include the variable segments (1-3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N-terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83% and 79% aa sequence identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, Flow Cytometry.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using FAB3660P	Applications	Species
Chuchu Gong, Zhengyan Gu, Xinkang Zhang, Qingqiang Xu, Guanchao Mao, Zhipeng Pei et al. HMSCs exosome‐derived miR‐199a‐5p attenuates sulfur mustard‐associated oxidative stress via the CAV1/NRF2 signalling pathway Journal of Cellular and Molecular Medicine 2023-08-01 [PMID: 37386746]
Nowak, J;Bentele, M;Kutle, I;Zimmermann, K;Lühmann, JL;Steinemann, D;Kloess, S;Koehl, U;Ro beta berg, W;Ahmed, A;Schaudien, D;Neubert, L;Kamp, JC;Kuehnel, MP;Warnecke, A;Schambach, A;Morgan, M; CAR-NK Cells Targeting HER1 (EGFR) Show Efficient Anti-Tumor Activity against Head and Neck Squamous Cell Carcinoma (HNSCC) Cancers 2023-06-13 [PMID: 37370779] (Flow Cytometry, Human)	Flow Cytometry	Human
Bing Yan, Ying Mu, Meiqi Cui, Long Liu Clinicopathological significance and prognostic implication of CD44 and its splice variants (v3 and v6) in colorectal cancer Translational Cancer Research 2020-02-01 [PMID: 35117466]
H Jin, HJ Kim NK Cells Lose Their Cytotoxicity Function against Cancer Stem Cell-Rich Radiotherapy-Resistant Breast Cancer Cell Populations International Journal of Molecular Sciences, 2021-09-06;22(17):. 2021-09-06 [PMID: 34502547] (Flow Cytometry, Human)	Flow Cytometry	Human
Meng Xia, Li-Jun Duan, Bi-Nan Lu, Yu-Zhou Pang, Zong-Ran Pang LncRNA AFAP1-AS1/miR-27b-3p/VEGF-C axis modulates stemness characteristics in cervical cancer cells Chinese Medical Journal 2021-09-05 [PMID: 34334630]
C Nicolazzo, F Loreni, S Caponnetto, V Magri, AR Vestri, R Zamarchi, A Gradilone, A Facchinett, E Rossi, E Cortesi, P Gazzaniga Baseline CD44v6-positive circulating tumor cells to predict first-line treatment failure in patients with metastatic colorectal cancer Oncotarget, 2020-11-10;11(45):4115-4122. 2020-11-10 [PMID: 33227095] (Bioassay, Human)	Bioassay	Human
Xiju Wang, Ronghua Wang, Shuya Bai, Si Xiong, Yawen Li, Man Liu et al. Musashi2 contributes to the maintenance of CD44v6+ liver cancer stem cells via notch1 signaling pathway Journal of Experimental & Clinical Cancer Research 2019-12-30 [PMID: 31888685]
KA Whelan, PM Chandramou, K Tanaka, M Natsuizaka, M Guha, S Srinivasan, DS Darling, Y Kita, S Natsugoe, JD Winkler, AJ Klein-Szan, RK Amaravadi, NG Avadhani, AK Rustgi, H Nakagawa Autophagy supports generation of cells with high CD44 expression via modulation of oxidative stress and Parkin-mediated mitochondrial clearance Oncogene, 2017-04-17;0(0):. 2017-04-17 [PMID: 28414310] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Okada , Toshihir, Nakamura , Teruo, Watanabe , Takayuki, Onoda , Naoyoshi, Ashida , Atsuko, Okuyama , Ryuhei, Ito , Ken-ichi Coexpression of EpCAM, CD44 variant isoforms and claudin-7 in anaplastic thyroid carcinoma. PLoS ONE, 2014-04-11;9(4):e94487. 2014-04-11 [PMID: 24727741] (Flow Cytometry, Human)	Flow Cytometry	Human
Krishnamachary B, Penet M, Nimmagadda S, Mironchik Y, Raman V, Solaiyappan M, Semenza G, Pomper M, Bhujwalla Z Hypoxia regulates CD44 and its variant isoforms through HIF-1alpha in triple negative breast cancer. PLoS ONE, 2012-08-28;7(8):e44078. 2012-08-28 [PMID: 22937154] (Flow Cytometry, Human)	Flow Cytometry	Human
McFadden C, Morgan R, Rahangdale S, Green D, Yamasaki H, Center D, Cruikshank W Preferential migration of T regulatory cells induced by IL-16. J. Immunol., 2007-11-15;179(10):6439-45. 2007-11-15 [PMID: 17982032] (Flow Cytometry, Human)	Flow Cytometry	Human
Show All 11 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	Flow	Human	02/08/2019	View Summary Application Flow Cytometry Sample Tested CD8 T lymphocytes Species Human Lot YAV0617061 Comments Comments Primary human CD8+ T-cells were isolated from PBMCs and activated with anti-CD3/anti-CD28 coated beads in culture.

Array FAB3660P

• CD44 Antibodies
• CD44 Antibody Pair
• CD44 ELISA Kits
• CD44 Lysates
• CD44 Protein
• CD44 ELISA
• CD44 Proteins and Enzymes