CD44 Antibody (2F10) [Allophycocyanin] - v6

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1-3). The standard or hematopoietic form, CD44H, does not include the variable segments (1-3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N-terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83% and 79% aa sequence identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, ICC.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using FAB3660A	Applications	Species
G Gallerani, T Rossi, M Valgiusti, D Angeli, P Fici, S De Fanti, E Bandini, C Cocchi, GL Frassineti, M Bonafè, F Fabbri CNA Profiling of Single CTCs in Locally Advanced Esophageal Cancer Patients during Therapy Highlights Unexplored Molecular Pathways Cancers, 2021-12-19;13(24):. 2021-12-19 [PMID: 34944989] (ICC, Human)	ICC	Human
A Turdo, A Glaviano, G Pepe, F Calapà, S Raimondo, ME Fiori, D Carbone, MG Basilicata, V Di Sarno, C Ostacolo, B Parrino, S Cascioferr, C Pecoraro, S Di Franco, D Bellavia, M Gaggianesi, V Veschi, M Lo Iacono, G Ganduscio, VD Pantina, LR Mangiapane, MR Bongiorno, R Alessandro, M Todaro, R De Maria, P Diana, P Campiglia, G Stassi Nobiletin and Xanthohumol Sensitize Colorectal Cancer Stem Cells to Standard Chemotherapy Cancers, 2021-08-04;13(16):. 2021-08-04 [PMID: 34439086] (Flow Cytometry, Human)	Flow Cytometry	Human
S Di Franco, P Bianca, DS Sardina, A Turdo, M Gaggianesi, V Veschi, A Nicotra, LR Mangiapane, M Lo Iacono, I Pillitteri, S van Hooff, F Martorana, G Motta, E Gulotta, VL Lentini, E Martorana, ME Fiori, S Vieni, MR Bongiorno, G Giannone, D Giuffrida, L Memeo, L Colarossi, M Mare, P Vigneri, M Todaro, R De Maria, JP Medema, G Stassi Adipose stem cell niche reprograms the colorectal cancer stem cell metastatic machinery Nature Communications, 2021-08-18;12(1):5006. 2021-08-18 [PMID: 34408135] (Flow Cytometry, Human)	Flow Cytometry	Human
Simone Di Franco, Barbara Parrino, Miriam Gaggianesi, Vincenzo Davide Pantina, Paola Bianca, Annalisa Nicotra et al. CHK1 inhibitor sensitizes resistant colorectal cancer stem cells to nortopsentin iScience 2021-06-25 [PMID: 34169240]
M Lepore Sig, V Grossi, S Di Franco, G Forte, V Disciglio, C Fasano, P Sanese, K De Marco, FC Susca, LR Mangiapane, A Nicotra, G Di Carlo, F Dituri, G Giannelli, G Ingravallo, G Canettieri, G Stassi, C Simone Pharmacological targeting of the novel &beta-catenin chromatin-associated kinase p38&alpha in colorectal cancer stem cell tumorspheres and organoids Cell Death & Disease, 2021-03-25;12(4):316. 2021-03-25 [PMID: 33767160] (Flow Cytometry, Human)	Flow Cytometry	Human
Luca Tirinato, Francesca Pagliari, Simone Di Franco, Elisa Sogne, Maria Grazia Marafioti, Jeanette Jansen et al. ROS and Lipid Droplet accumulation induced by high glucose exposure in healthy colon and Colorectal Cancer Stem Cells Genes & Diseases 2020-12-01 [PMID: 33335962]
C Pereira, D Ferreira, N Mendes, PL Granja, GM Almeida, C Oliveira Expression of CD44v6-Containing Isoforms Influences Cisplatin Response in Gastric Cancer Cells Cancers (Basel), 2020-04-02;12(4):. 2020-04-02 [PMID: 32252293] (Flow Cytometry, Human)	Flow Cytometry	Human
V Veschi, LR Mangiapane, A Nicotra, S Di Franco, E Scavo, T Apuzzo, DS Sardina, M Fiori, A Benfante, ML Colorito, G Cocorullo, F Giuliante, C Cipolla, G Pistone, MR Bongiorno, A Rizzo, CM Tate, X Wu, S Rowlinson, LF Stancato, M Todaro, R De Maria, G Stassi Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant Oncogene, 2019-10-07;0(0):. 2019-10-07 [PMID: 31591478] (Flow Cytometry, Human)	Flow Cytometry	Human
Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma Mediators Inflamm, 2016-08-28;2016(0):9343460. 2016-08-28 [PMID: 27647953] (Flow Cytometry, Human)	Flow Cytometry	Human
Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis. Cell Stem Cell, 2014-03-06;14(3):342-56. 2014-03-06 [PMID: 24607406] (Flow Cytometry, Human)	Flow Cytometry	Human
Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer. J. Exp. Clin. Cancer Res., 2012-03-23;31(0):26. 2012-03-23 [PMID: 22444159] (Flow Cytometry, Human)	Flow Cytometry	Human
Show All 11 Publications. Collapse Publications.

Array FAB3660A

• CD44 Antibodies
• CD44 Antibody Pair
• CD44 ELISA Kits
• CD44 Lysates
• CD44 Protein
• CD44 ELISA
• CD44 Proteins and Enzymes