![Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Analysis using the FITC conjugate of NB100-63790. Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD42b:FITC.](http://images.novusbio.com/fullsize/CD42b-GPIb-alpha-Antibody-AK2-Flow-Cytometry-NB100-63790-img0011.jpg "Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Analysis using the FITC conjugate of NB100-63790. Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD42b:FITC.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, IP |
| Clone | AK2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Additional Information | Clone AK2 was used by HLDA to establish CD designation. |
| Immunogen | Made to Human CD42b |
| Specificity | Recognises the human CD42b cell surface antigen, also known as platelet glycoprotein GP1B. CD42b is expressed by platelets and megakaryocytes. Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets (1). |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GP1BA |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 69 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets with Mouse anti Human CD42b.](http://images.novusbio.com/fullsize/CD42b-GPIb-alpha-Antibody-AK2-Flow-Cytometry-NB100-63790-img0001.jpg "Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets with Mouse anti Human CD42b.")
![Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets.](http://images.novusbio.com/fullsize/CD42b-GPIb-alpha-Antibody-AK2-Flow-Cytometry-NB100-63790-img0004.jpg "Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets.")
![Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets.](http://images.novusbio.com/fullsize/CD42b-GPIb-alpha-Antibody-AK2-Flow-Cytometry-NB100-63790-img0005.jpg "Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets.")
![Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD42b: RPE.](http://images.novusbio.com/fullsize/CD42b-GPIb-alpha-Antibody-AK2-Flow-Cytometry-NB100-63790-img0009.jpg "Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD42b: RPE.")
![Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Analysis using the Biotin conjugate of NB100-63790. Staining of human peripheral blood platelets.](http://images.novusbio.com/fullsize/CD42b-GPIb-alpha-Antibody-AK2-Flow-Cytometry-NB100-63790-img0010.jpg "Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Analysis using the Biotin conjugate of NB100-63790. Staining of human peripheral blood platelets.")
![Immunoprecipitation: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - After labeling the surface proteins of the CHO a beta IX cells with biotin, the GPIbIX complex was immunoprecipitated using the MoAb panel. The presence of the GPIba and GPIX native proteins was shown on the surface of the CHO a beta IX cells. Lane 1 shows the components immunoprecipitated using AK3, lane 2 with AK2, and lane 3 with SZ2. After cleavage with mocarhagin, AK3 precipitated a 70-kD GPIba component and the intact GPIX protein from the surface of the cells (lane 4). AK2 (lane 5) and SZ2 (lane 6) were able to precipitate a 40-kD GPIba component from the supernatant collected after the cleavage step.](http://images.novusbio.com/fullsize/CD42b-GPIb-alpha-Antibody-AK2-Immunoprecipitation-NB100-63790-img0003.jpg "Immunoprecipitation: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - After labeling the surface proteins of the CHO a beta IX cells with biotin, the GPIbIX complex was immunoprecipitated using the MoAb panel. The presence of the GPIba and GPIX native proteins was shown on the surface of the CHO a beta IX cells. Lane 1 shows the components immunoprecipitated using AK3, lane 2 with AK2, and lane 3 with SZ2. After cleavage with mocarhagin, AK3 precipitated a 70-kD GPIba component and the intact GPIX protein from the surface of the cells (lane 4). AK2 (lane 5) and SZ2 (lane 6) were able to precipitate a 40-kD GPIba component from the supernatant collected after the cleavage step.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")