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CD40/TNFRSF5 Antibody (82111) [Phycoerythrin]

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Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD40/TNFRSF5 PE-conjugated Monoclonal Antibody (Catalog # FAB6321P) and Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F). Quadrant markers were set based on control antibody staining (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD40/TNFRSF5 PE-conjugated Monoclonal Antibody (Catalog # FAB6321P) and Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # ...read more
PBMC lymphocytes were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD40/TNFRSF5 PE‑conjugated Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
82111
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB6321U-100UG
Alexa Fluor 405
FAB6321V-100UG
Alexa Fluor 488
FAB6321G-100UG
Alexa Fluor 532
FAB6321X
Alexa Fluor 594
FAB6321T-100UG
Alexa Fluor 647
FAB6321R-100UG
Alexa Fluor 700
FAB6321N-100UG
Alexa Fluor 750
FAB6321S-100UG
Allophycocyanin
FAB6321A
Allophycocyanin/Cy7
FAB6321APCCY7
Biotin
FAB6321B
CoraFluor 1
FAB6321CL1
DyLight 350
FAB6321D
DyLight 405
FAB6321E
DyLight 488
FAB6321K
DyLight 550
FAB6321L
DyLight 594
FAB6321M
DyLight 650
FAB6321W
DyLight 680
FAB6321Y
DyLight 755
FAB6321Z
FITC
FAB6321F
HRP
FAB6321H
Janelia Fluor 525
FAB6321JF525
Janelia Fluor 549
FAB6321I
Janelia Fluor 585
FAB6321JF585
Janelia Fluor 635
FAB6321JF635
Janelia Fluor 646
FAB6321J
Janelia Fluor 669
FAB6321JF669
PE/Atto594
FAB6321PEATT594
PE/Cy5.5
FAB6321PECY55
PE/Cy7
FAB6321PECY7
PerCP
FAB6321C
Unconjugated
MAB6321-100
mFluor Violet 450 SE
FAB6321MFV450
mFluor Violet 500 SE
FAB6321MFV500
mFluor Violet 610 SE
FAB6321MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB6321-100
Carrier Free
FAB6321A
Carrier Free
MAB6321-SP
Carrier Free
MAB6321-100
Carrier Free
MAB6321-500
Carrier Free
FAB6321V-100UG
Carrier Free
FAB6321U-100UG
Carrier Free
FAB6321T-100UG
Carrier Free
FAB6321S-100UG
Carrier Free
FAB6321R-100UG
Carrier Free
FAB6321N-100UG
Carrier Free
FAB6321G-100UG
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CD40/TNFRSF5 Antibody (82111) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD40/TNFRSF5
Glu21-Arg193
Accession # P25942
Specificity
Detects human CD40/TNFRSF5 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta , rhNGF R, rhOPG, rhRANK, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse CD40.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CD40
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB6321P in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD40/TNFRSF5 Antibody (82111) [Phycoerythrin]

  • B cell surface antigen CD40
  • B-cell surface antigen CD40
  • Bp50B cell-associated molecule
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • CD40 type II isoform
  • CD40
  • CD40L receptor
  • CDw40
  • MGC9013
  • nerve growth factor receptor-related B-lymphocyte activation molecule
  • p50
  • TNFRSF5
  • TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
  • tumor necrosis factor receptor superfamily member 5
  • tumor necrosis factor receptor superfamily, member 5

Background

CD40, also known as TNFRSF5, is a 45-50 kD type I transmembrane glycoprotein that belongs to the TNF receptor superfamily. Mature human CD40 is 257 amino acids (aa) in length, and consists of a 173 aa extracellular domain (ECD), a 22 aa transmembrane segment and a 62 aa cytoplasmic region. The ECD contains four cysteine-rich repeats, the second of which is considered crucial to ligand binding. Over aa 21-193, human CD40 shares 58% aa sequence identity with mouse CD40. Although not ubiquitously expressed, cells known to constitutively or inducibly express CD40 are large in number and include basal layer keratinocytes, adipocytes, CD8+ T cells, fibrocytes (circulating stem cells), monocytes, eosinophils, dendritic cells, pancreatic duct epithelium, astrocytes, smooth muscle cells, fibroblasts, vascular endothelial cells and B cells. There is at least one alternative splice form that shows a 38 aa substitution for aa 166-277, generating a soluble molecule. There are two "ligands" for CD40, the first being CD154/CD40L, and the second Borrelia, a spirochete that causes Lyme disease. CD40 ligation by CD154 has many outcomes, some of which are cell-specific. Ligation of CD40 on B cells (with IL-4) induces IgE production, while bone marrow stromal cell activation via CD40 promotes OPG secretion with concomitant bone formation. In addition, stimulation of CD40 on keratinocytes results in IL-8 and RANTES expression with subsequent leukocyte infiltration, while adipocyte activation via CD40 promotes local M1 polarization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD40/TNFRSF5 Antibody (FAB6321P)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Chip Cytometry, Flow Cytometry.


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(1)
Flow Cytometry
(1)
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(2)
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Product General Protocols

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FAQs for CD40/TNFRSF5 Antibody (FAB6321P). (Showing 1 - 2 of 2 FAQs).

  1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
    • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
  2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
    • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CD40
Uniprot