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CD300c Antibody

Images

 
Western Blot: CD300c/LMIR2 Antibody [NBP1-84432] - Analysis in control (vector only transfected HEK293T lysate) and CD300C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian ...read more
Immunohistochemistry-Paraffin: CD300c/LMIR2 Antibody [NBP1-84432] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CD300c Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PWLRDFHDPIVEVEVSVFPAGTTTASSPQSSMGTSGPPTKLPVHTWPSVTRKDSPEPSPHPGSLFSNVR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD300C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CD300c Recombinant Protein Antigen (NBP1-84432PEP)
Publications
Read Publication using
NBP1-84432 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CD300c Antibody

  • CD300c antigenCMRF35 antigen
  • CD300c molecule
  • CD300c
  • CLM6
  • CLM-6
  • CMRF35 leukocyte immunoglobulin-like receptor
  • CMRF35
  • CMRF-35
  • CMRF35A leukocyte immunoglobulin-like receptor
  • CMRF-35A
  • CMRF35ACMRF35A1
  • CMRF35CMRF35-A1
  • CMRF35-like molecule 6
  • IGSF16
  • IgSF16
  • IGSF16CD300 antigen-like family member C
  • Immunoglobulin superfamily member 16
  • LIR

Background

CD300C - CD300C antigen

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD300c Antibody (NBP1-84432)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CD300c Antibody (NBP1-84432). (Showing 1 - 1 of 1 FAQ).

  1. Why are there different molecular weight bands in western blot images for two of your antibodies (NBP1-84432 and H00010871-B01P) and overexpression lysate NBL1-08931?
    • A variety of band patterns can be expected when detecting CD300C. This protein has multiple glycosliation post-translational modifications that will change its molecular weight, and which vary in different experimental conditions. If you look at the uniprot link listed below, you can view more information on the specific PTMs for CD300C:https://www.uniprot.org/uniprot/Q08708#sequencesWe can be assured that these varying band patterns are indeed detection of CD300C by the absence of bands in the negative control. Any non-specific binding would show in both the negative and positive control, which is not the case here.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD300C