![Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Western blot analysis of CD3G on mouse thymus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Western-Blot-NBP2-75435-img0006.jpg "Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Western blot analysis of CD3G on mouse thymus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC, IP |
| Clone | JB38-29 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human CD3 gamma aa 133-182 / 182. (SwissProt: P09693 Human; SwissProt: P11942 Mouse) |
| Localization | Cell membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CD3G |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot Ets-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ets-1_AF7284_Western_Blot_11854.jpg)
![Immunohistochemistry Ets-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ets1_AF7284_Immunohistochemistry_22698.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for CD3 gamma Antibody (NBP2-75435)Find related products by research area.
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| Gene Symbol | CD3G |
![Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human spleen tissue using anti-CD3G antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Immunohistochemistry-Paraffin-NBP2-75435-img0003.jpg "Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human spleen tissue using anti-CD3G antibody. Counter stained with hematoxylin.")
![Flow Cytometry: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of Jurkat cells with CD3G antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Flow-Cytometry-NBP2-75435-img0001.jpg "Flow Cytometry: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of Jurkat cells with CD3G antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of CD3G on mouse thymus tissue lysate using anti-CD3G antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Western-Blot-NBP2-75435-img0004.jpg "Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of CD3G on mouse thymus tissue lysate using anti-CD3G antibody at 1/500 dilution.")
![Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Western blot analysis of CD3G on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Western-Blot-NBP2-75435-img0005.jpg "Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Western blot analysis of CD3G on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human tonsil tissue using anti-CD3G antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Immunohistochemistry-Paraffin-NBP2-75435-img0002.jpg "Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human tonsil tissue using anti-CD3G antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")