![Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Western blot analysis of CD3G on mouse thymus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Western-Blot-NBP2-75435-img0006.jpg "Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Western blot analysis of CD3G on mouse thymus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC, IP |
| Clone | JB38-29 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human CD3 gamma aa 133-182 / 182. (SwissProt: P09693 Human; SwissProt: P11942 Mouse) |
| Localization | Cell membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CD3G |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot Ets-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ets-1_AF7284_Western_Blot_11854.jpg)
![Immunohistochemistry Ets-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ets1_AF7284_Immunohistochemistry_22698.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for CD3 gamma Antibody (NBP2-75435)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CD3G |
![Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human spleen tissue using anti-CD3G antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Immunohistochemistry-Paraffin-NBP2-75435-img0003.jpg "Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human spleen tissue using anti-CD3G antibody. Counter stained with hematoxylin.")
![Flow Cytometry: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of Jurkat cells with CD3G antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Flow-Cytometry-NBP2-75435-img0001.jpg "Flow Cytometry: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of Jurkat cells with CD3G antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of CD3G on mouse thymus tissue lysate using anti-CD3G antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Western-Blot-NBP2-75435-img0004.jpg "Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of CD3G on mouse thymus tissue lysate using anti-CD3G antibody at 1/500 dilution.")
![Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Western blot analysis of CD3G on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Western-Blot-NBP2-75435-img0005.jpg "Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Western blot analysis of CD3G on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human tonsil tissue using anti-CD3G antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD3-gamma-Antibody-JB38-29-Immunohistochemistry-Paraffin-NBP2-75435-img0002.jpg "Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human tonsil tissue using anti-CD3G antibody. Counter stained with hematoxylin.")
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")